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Questions tagged [proteins]

For questions about proteins. Proteins are biopolymers consisting primarily of polycondensated amino acids.

2 votes
1 answer
92 views

By what mechanism does amylase catalyze hydrolysis?

I've recently been learning bits and pieces of starch metabolism, and am wondering if anyone could explain exactly what is going on when alpha or beta amylase "cleaves" the 1-4 glycosidic ...
Matthew Finger's user avatar
2 votes
2 answers
145 views

Does the word "restraint" have any special meaning in the case of protein or biochemistry?

The following screenshot is taken from the book The Encyclopedia of Physical Science & Technology, volume: Biochemistry, Edition: 3rd, Page-197. The text says: FIGURE 3 Schematic representation ...
user avatar
2 votes
0 answers
71 views

Is there any special meaning to the angle between two peptide planes?

Take a look at the following image: Is there any special meaning to the angle between two peptide planes (marked with red arrow heads)? If so, why isn't a specific name (like phi, psi, etc.) given?
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1 vote
0 answers
31 views

Why is AChE inhibited by triflates not susceptible to reactivation?

According to a report, certain esters of trifluoromethanesulfonic acid are capable of acting as irreversible inhibitors of acetylcholinesterase. Also, 3-PAM, while capable of reactivating AChE ...
user73910's user avatar
  • 1,264
0 votes
1 answer
95 views

Is it possible to replace 3-mercaptopropionic acid with 2-mercaptoethanol to solubilize avian eggshell membrane to soluble eggshell membrane protein?

Is it possible to replace 3-mercaptopropionic acid with 2-mercaptoethanol to solubilize avian eggshell membrane (ESM) to soluble eggshell membrane protein (SEP)? Most literature suggested the use of ...
Aina Razali's user avatar
2 votes
0 answers
103 views

Protein/polypeptide nomenclature, residue number superscripted or not?

When referring to a specific residue number of a given amino acid, should the residue number be superscripted or simply affixed? For example, When referring to a Tryptophan residue at the 147th ...
sat0ri's user avatar
  • 161
0 votes
0 answers
71 views

Do whey proteins precipitate with heat?

I am currently studying about the biochemistry of yoghurt and when reading about the effect of heat in milk proteins specifically whey, my book says that heat can denature whey proteins. So after the ...
CaptainAmerica Whyso's user avatar
1 vote
0 answers
92 views

How come in biuret test the copper sulfate doesn't react with the sodium hydroxide?

As I understand the color change in the biuret test is due to the formation of a complex between the peptide (with more than 2 peptide bonds) and the $\ce{Cu^2+}$ ion, from $\ce{CuSO4}$. But before ...
CaptainAmerica Whyso's user avatar
8 votes
1 answer
311 views

Why is collagen fibre autofluorescent?

Why is collagen fibre autofluorescent? Proteins with increased amount of trp, tyr, phe tend to fluoresce but I don't think collagen fibre has increased percentage of any of them. Some say collagen ...
Yushi Li's user avatar
  • 197
1 vote
3 answers
407 views

Why do hydrogen bonds between atoms of the polypeptide backbone, form both helix and pleated structures, instead of only one structural type?

Proteins have segments of their polypeptide chain/chains that can be repeatedly coiled or folded into helix and pleated structures, respectively. This is due to hydrogen bonds between partially ...
Growing6884's user avatar
0 votes
2 answers
567 views

Why are proteins made up of alpha amino acids and not beta amino acids?

Why are proteins made up of alpha amino acids and not beta amino acids? Or gamma amino acids? My idea on this would be that a world with beta or gamma amino acids would be too complicated? Is that ...
saturn20005252's user avatar
-3 votes
1 answer
49 views

what is the meaning by 'species' of glycine? [closed]

The question ask for the species of the (i) glycine hydrochloride (ii) glycine titrated with NaOH. When they mentioned 'species', what did they mean by it?
Nku's user avatar
  • 1
1 vote
0 answers
41 views

How to calculate absolute charge of a protein [closed]

My understanding is that hydrogen bonds formed by proteins require the NH2 / COOH to be neutral. Hence to find out when the hydrogen bonds are the strongest will depend on the absolute charge of the ...
TheRavenSpectre's user avatar
6 votes
2 answers
244 views

How is geometry optimization of small molecules different from the protein folding problem?

My layman's understanding is that finding the ground state geometry of small molecules is "hard". I don't have a good sense of how hard though. I suppose there are applications where methods ...
theQman's user avatar
  • 169
3 votes
1 answer
103 views

Contaminants in Ion Exchange Chromatography significance?

I am a high school senior and decided to do a project on the essential amino acid composition of varying vegan protein mixtures. I have run into a problem concerning protein purification. One of the ...
I do not want to put my name's user avatar

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