All Questions
Tagged with proteins purification
7
questions
1
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0
answers
52
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How does ethanol separates salts from proteins [closed]
Grad student here messing around with famed TRIzol isolation protocol and looking to optimize the protein yield at the end. There are more direct ways to get protein from a sample but I like getting ...
- 111
-1
votes
1
answer
46
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How should I interpret and present my total protein amount? [closed]
I have a problem. I have purified a protein with method A and then B. After method A I have a protein concentration of 20 mg/ml and after method B a concentration of 18 mg/ml. Method B is dialysis and ...
- 25
1
vote
0
answers
29
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Choice of buffer pH to avoid aggregation
I want to purify IgG which has a theoretical pI of 6.4-9.0 with an anion exchange column. I also want to keep the pH of my buffers as low as possible and "outside" the range of the pI to prevent ...
- 29
5
votes
1
answer
1k
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How to make a sephadex slurry
I want to purify crude alpha-amylase with column chromatography. I am using a spehadex 75, but for some reason I can't find any information on how to make the slurry.
I can quickly find a lot of ...
- 51
2
votes
1
answer
13k
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Why is % yield in a purification table measured using activity instead of total protein?
I'm trying to understand why % yield is measured with activity instead of total protein in mg. According to my biochemistry notes, activity is "1.0 units of enzyme activity = 1.0 μmol of substrate ...
- 65
6
votes
1
answer
3k
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Mechanism of protein precipitation with TCA
In practice, TCA (trichloroacetic acid) is widely used for quantitative protein precipitation, but so far I have failed to discover the exact mechanism of TCA action. I highly doubt it's just the ...
6
votes
1
answer
233
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Protein purification with Cobalt
Cobalt exhibits a more specific interaction with histidine tags, resulting in less nonspecific interaction than nickel. For this reason, cobalt is the preferred divalent cation for purifying His-...
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