All Questions
Tagged with proteins experimental-chemistry
6
questions
0
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1
answer
111
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Testing whether an unknown powder is wheat gluten (at home)?
Last week, I ordered wheat gluten to make seitan. I received some powder and attempted a recipe, which mixes 100g of water with 100g of gluten to form a sticky dough. However in my case, I only got a ...
- 109
-3
votes
1
answer
49
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what is the meaning by 'species' of glycine? [closed]
The question ask for the species of the (i) glycine hydrochloride (ii) glycine titrated with NaOH. When they mentioned 'species', what did they mean by it?
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3
votes
1
answer
103
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Contaminants in Ion Exchange Chromatography significance?
I am a high school senior and decided to do a project on the essential amino acid composition of varying vegan protein mixtures. I have run into a problem concerning protein purification. One of the ...
0
votes
0
answers
87
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Developing a lab to identify the concentration of casein in organic milk
I am currently attempting to create a feasible lab design that will enable me to identify the concentration of casein protein in organic milk without having to use spectroscopy. Thus far I have ...
- 640
3
votes
1
answer
3k
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Gel electrophoresis separates proteins on the basis of what property?
In SDS-PAGE for separation of proteins, SDS is an ionic detergent that coats the unfolded protein to mask its native charge and give at a uniform charge to length ratio. Since the length of the ...
- 422
6
votes
1
answer
3k
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Mechanism of protein precipitation with TCA
In practice, TCA (trichloroacetic acid) is widely used for quantitative protein precipitation, but so far I have failed to discover the exact mechanism of TCA action. I highly doubt it's just the ...
