All Questions
Tagged with proteins crystal-structure
9
questions
10
votes
1
answer
726
views
Why does this lysozyme powder not look crystalline?
I am very new to protein crystallography and visualization. I have been asked to take photos through an optical microscope of the lysozyme powder directly as-is from the supplier. I suspended some of ...
- 111
2
votes
2
answers
383
views
Lysozyme crystals versus NaCl
If someone has some experience with lysozyme crystals I would be interested in knowing whether any of the images below seem likely to be lysozyme. Solutions were made with powdered lysozyme ($\pu{50 ...
- 994
0
votes
1
answer
120
views
What do X-ray diffraction and NMR tell about the Proteins? [closed]
I have gone through a simple idea that proteins have different conformations; it is a dynamic system. Moreover, whenever we do ensemble experiments, so we get an average picture. There are some ...
- 1,186
-1
votes
1
answer
474
views
How to visualize a crystal from a unit cell PDB and how to draw the unit cell's axes? [closed]
I am looking at the following PDB experimental data:
Length (Å) Angle (°)
a = 155.72 α = 90.00
b = 155.72 β = 90.00
c = 106.16 γ = 120.00
I find it hard to ...
- 924
5
votes
1
answer
379
views
Alternative to Hanging Drop Crystallization
Forgive my ignorance if I am way off, but I was having a look at the crystallization of a number of different substances (e.g. proteins) through the hanging drop vapour diffusion method.
The examples ...
- 151
4
votes
2
answers
3k
views
Why are there no hydrogen atoms in the crystal structure of a biomolecule?
I am taking a physics and chemistry at the nanoscale class at my university and for a project I must learn how to use Visual Molecular Dynamics (VMD). One of the models involved in this project is apo-...
2
votes
0
answers
140
views
RCSB PDB - how to choose between multiple copies of the same protein?
I want to map the location of missense mutations on a protein, SERPINB3. I intend to do this using PyMol and download the protein structure from RCSB PDB. However, ...
- 383
7
votes
1
answer
841
views
Protein Data Bank: Asymmetric Unit/Biological Assembly
For a schoolproject I have to interpret the 3D structure of an imatinib-BCR ABLC kinase complex. When I visited the PDB website I downloaded the PDB file corresponding to this complex. Once I opened ...
- 463
7
votes
2
answers
1k
views
What is the difference between crystal structures of proteins, organic and inorganic materials?
From the definition of crystal and the main differences between crystalline and amorphous material, it is known that crystal formation requires ordered bonding between atoms or molecules.
How can ...
- 497