All Questions
Tagged with proteins analytical-chemistry
6
questions
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40
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What is the industrial alternative to dialysis?
I want to separate a recombinant protein from imidazole. Both of them are in a solution of pH 8. I was thinking of using ion exchange chromatography with cationite: the imidazole being positively ...
- 109
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1
answer
111
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Testing whether an unknown powder is wheat gluten (at home)?
Last week, I ordered wheat gluten to make seitan. I received some powder and attempted a recipe, which mixes 100g of water with 100g of gluten to form a sticky dough. However in my case, I only got a ...
- 109
2
votes
1
answer
88
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What can I do if a peptide won't go in solution in a biological assay?
I have working on the realisation of on assay, that is intended to examine the activity of a protein. The assay works in a way, in which the product of the target enzyme is transfered by a support ...
- 799
2
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How can DI water inhibit peroxidase activity?
I'm troubleshooting an IHC staining issue, and according to Thermo-Fischer's website, "Deionized water can sometimes contain peroxidase inhibitors that can significantly impair enzyme activity.&...
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2
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373
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Can I normalize my data in order to compare it?
I have three chromatograms of the same eluted protein but at different concentrations, since it got diluted during purification process) and hence peak maximums at very different absorbances.
The ...
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6
votes
1
answer
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Is there a chemical test for gluten?
I am looking for a chemical test for the presence of gluten: however, having looked online for such a thing, all the results seem to be about 'home testing kits' (presumably for people with a gluten ...
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