All Questions
Tagged with proteins chemical-biology
7
questions
3
votes
0
answers
35
views
Histidine-Boc deprotectection
A question on Fmoc-solid phase peptide synthesis (SPPS). Is Boc-protected Histidine too labile? Merck's website shows Boc is labile to treatment with Piperidine. Anyone with such experience?
- 157
4
votes
1
answer
164
views
What is the maximum length of peptide which considered to be hard for synthesis by the current technology?
I am a molecular biologist and work with recombinant proteins. I am wondering what the cut point, in terms of length, is at which I should produce the peptide using heterologous expression rather than ...
- 131
5
votes
1
answer
658
views
SARS-CoV - relative size of the spike protein
I am taking an online course in image analysis and have been asked to use the attached image to determine the size of the SARS-S protein of the SARS-CoV relative to the total of its proteins. However, ...
- 181
0
votes
1
answer
205
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Turn Alpha Helix into Beta Pleat
Beta sheets have the peptide backbone parallel whilst the alpha helix has a curled backbone.
But the thing I can't get around my head is why would the same protein always fold into one of these shapes?...
- 1,584
2
votes
2
answers
191
views
Purifying cysteine containing peptides
I would like to purify my peptide having cysteine residue by using HPLC. It is known that thiol containing peptides undergo oxidation and form disulfide linkages. In order to prevent it I used proper ...
- 157
4
votes
2
answers
583
views
How to measure distance along solvent-accessible surface of protein complex in PDB file?
I am looking for a straightforward way to measure the distance between two active sites in a protein complex (PDB file).
I need the distance along the solvent-accessible surface of the protein and not ...
- 313
5
votes
1
answer
235
views
Thermal stability of charged and uncharged alpha helices (the concept of alpha-helix capping)
I just learned that charged alpha helices are less thermally stable than uncharged ones. The only difference I see between them would be a larger dipole moment in the charged helix, but how would this ...
- 662