Questions tagged [biochemistry]
This tag is for questions concerning biochemical methods (e.g. electrophoresis) or those concerning biochemical mechanisms or research. Do not use this tag if your question is merely about compounds often used in areas related to biochemistry or associated with these. These may fall under organic chemistry or the appropriate compound’s functional groups’ tags.
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Could this glassy "Amber-Like" polymer used to store DNA be exposed to sunlight?
An interesting article, that I came across today, talked about the ongoing research into storing DNA at room temperature essentially with glassy "amber-like" polymers. Before talking about ...
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Drug dosage determination using dynamical systems
I am a Mathematical Biology student, and recently I have read about various approaches, such as Ordinary Differential Equations (ODEs), used to determine the exact drug dosage and treatment sessions ...
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What role does CaCl2 play in a gelation medium?
To produce sodium alginate beads coated with chitosan, following procedure was undertaken:
Sodium alginate was dissolved in distilled water at a concentration of 4% (...) The gelation medium was ...
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Does oxygen bind to Fe2+ or Fe3+ in heme of hemoglobin? And why?
My biochemistry teacher said that only $\ce{Fe^2+}$ in hemoglobin can bind to oxygen, while $\ce{Fe^3+}$ cannot.
I researched this and found an article that states that it is $\ce{Fe^3+}$ that binds ...
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What does ‘D’ in GADP (glyceraldehyde 3-phosphate) stand for?
Wikipedia says glyceraldehyde-3-phosphate can be abbreviated as G3P, GA3P, GADP, GAP, TP, GALP or PGAL, and many resources seem to use GADP as its abbreviation.
I have no idea where ‘D’ came from. ...
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Is nitroglycerin synthesis within human digestive system from eating soap possible?
Could you make nitroglycerin by eating large quantities of pure glycerine soap? A quick google search told me flatus are 59% nitrogen, but I couldn't find anyone else with my exact question (which ...
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why is alpha-linolenic acid "alpha"?
I'm learning about how fatty acid names are formed. In the process, I noticed a molecule called alpha-linolenic acid. There's a similar molecule called linoleic acid. Both have 18 carbons, but alpha-...
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Why does tert-butyl alcohol smell like camphor?
Industrial alcohol sold in Taiwan often have a pronounced smell that resembles camphor. When I checked the ingredients at the back, it included tert-butyl alcohol $\ce{(CH_3)_3COH}$, which Wikipedia ...
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How is it known that proteins are polymers of amino acids?
I read the following here.
In 1902, Emil Fischer and Frank Hofmeister independently proposed that proteins are formed from many amino acids, whereby bonds are formed between the amino group of one ...
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Protein quaternary structure in ViewLite
I want to represent the octameric structure of a metalloprotein in the ViwerLite program. I loaded the PDB file related to the protein, but the progranul only displays a monomeric structure (a single ...
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More potent caffeine analogs (in silico study)
Is it possible to do a Structure Activity Relationship for caffeine given that its structure is similar to that of adenosine? Maybe more potent analogues can be made using caffeine as lead compound?
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Assessing stability of protein used for interaction experiments after changing buffer from tris to HEPES
I have a protein with a theoretical $\mathrm{pI}~9.0$ currently stored in $\pu{25 mM}$ tris and $\pu{250 mM}$ $\ce{NaCl}$ $(\mathrm{pH}~8)$ at $\pu{−80 °C}.$ I need to do experiments in HEPES at $\...
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What is the best alternative to dialysis?
What is the best alternative to dialysis? I want to "design" at a purely theoretical level an industrial installation for the purification of a recombinant protein and dialysis is not ...
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What is the industrial alternative to dialysis?
I want to separate a recombinant protein from imidazole. Both of them are in a solution of pH 8. I was thinking of using ion exchange chromatography with cationite: the imidazole being positively ...
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Why can't the fructose monomer in sucrose be α-fructose?
In all academic sources, sucrose is identified as α−glucose (1-->2) β−fructose. However, I cannot find any explanation anywhere as to why the fructose monomer has to be in the β configuration. ...
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