All Questions
9
questions
2
votes
0
answers
25
views
Reason for differences in AChE aging/reactivation kinetics between neutral and charged organophosphorus inhibitors
According to the referenced papers, acetylcholinesterase that has been inhibited by an organophosphate possessing thiocholine as its leaving group is more susceptible to reactivation by oxime ...
- 1,264
3
votes
1
answer
105
views
Effect of presence of competitive inhibitor on observed reaction constant
Suppose there is a solution of enzyme, its substrate, a competitive inhibitor, and a suicide inhibitor. The reaction rate constant for the reaction of the suicide inhibitor with the enzyme is known. ...
- 1,264
3
votes
1
answer
174
views
Effect of competitive inhibitor on substrate inhibition
In an enzyme that undergoes substrate inhibition, how would the presence of a competitive inhibitor affect said substrate inhibition? Would the substrate concentration at which substrate inhibition ...
- 1,264
2
votes
0
answers
39
views
How are enzyme inhibition constants assayed for "multi-inhibitors"?
Suppose there is a suicide inhibitor of an enzyme that reacts with the enzyme to form an inactive enzyme and another product. This "Another product", however, is capable of acting as a ...
- 1,264
3
votes
0
answers
34
views
Differences in AChE inhibition kinetics between VX and VR
According to the referenced paper, VR has an AChE inhibition reaction rate constant almost 4 times that of VX. Interestingly, its reaction rate constant for aging of the enzyme is less than that of VX,...
- 1,264
4
votes
2
answers
828
views
Why does denaturation of albumen protein cause pH of solution to increase?
I'm interested in the denaturation of proteins by alcohols, specifically by ethanol.
I have devised a simple experiment in which I inject solutions of ethanol (of variable concentrations) into ...
- 151
2
votes
0
answers
81
views
Calcium concentration in NMDARs e Nitric Oxide Synthase
My question is related to calcium $\ce{Ca^{2+}}$ concentration in NMDA receptors and nitric oxide synthase. I've found a very simple model that describes how the time change of calcium can be related ...
3
votes
1
answer
188
views
Effect of His-tag on enzyme activity
In my biochemistry laboratory class, I designed an experiment to study the effect of a His-tag on enzyme activity. First, I measured the activity of the His-tagged enzyme. Then, I cut off the His-tag (...
- 455
2
votes
0
answers
53
views
What are some technologies/biosensors for real time kinetic analysis of reactions? [closed]
I am in a lab that has used surface plasmon resonance (SPR) and ELISA very heavily over the past few years, and we are thinking of getting a new machine. I'm wondering what other technologies exist ...
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