All Questions
Tagged with proteins chromatography
7
questions
2
votes
0
answers
106
views
Right conductivity of protein sample for Protein A purification of human IgG?
A sample from a cell culture has some IgG and this is to be purified using protein A chromatography. Based on the chromatography handbook from GE-Healthcare/Cytiva, the protein sample should have &...
- 119
3
votes
1
answer
138
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Why can DAP be used to elute a protein in affinity chromatography?
A little background: as part of my bioinformatics degree I have to take Protein Chemistry course, but I miss chemistry basics (have CS background), so that's why I'm asking the following question.
We ...
- 139
3
votes
2
answers
810
views
What dictates cathode vs anode nomenclature use?
what was the justification for the use the cathode and anode terminology in IEF?
There is no redox taking place in IEF, just proteins interacting with an electric field
1
vote
2
answers
373
views
Can I normalize my data in order to compare it?
I have three chromatograms of the same eluted protein but at different concentrations, since it got diluted during purification process) and hence peak maximums at very different absorbances.
The ...
- 25
3
votes
1
answer
2k
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How to pool fractions after chromatography?
I used chromatography to purify my protein and now I have fractions with different concentrations ranging from 1-16 mg/ml. Should I pool all these fractions or just the ones with high concentration (...
- 31
5
votes
1
answer
1k
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How to make a sephadex slurry
I want to purify crude alpha-amylase with column chromatography. I am using a spehadex 75, but for some reason I can't find any information on how to make the slurry.
I can quickly find a lot of ...
- 51
6
votes
1
answer
233
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Protein purification with Cobalt
Cobalt exhibits a more specific interaction with histidine tags, resulting in less nonspecific interaction than nickel. For this reason, cobalt is the preferred divalent cation for purifying His-...
- 61