Questions tagged [genetics]
Genetics is the branch of biology that deals with the transmission and variation of inherited characteristics.
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Use of plasmid pXen5 for transposon screening
I would like to use the plasmid pXen5 (by Xenogen) for a transposon screen.
It contains two inverted repeat sequences, with Luciferase, Kanamycin, and the transposase itself in between. (It's tn1409).
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Post-Translational Modification in insulin production
When searching "How is insulin produced commercially" on Google, most results simply say something along the lines of "The insulin gene is inserted into a bacteria, which then express ...
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What backbone to use for Helper dependent adenovirus (HDAd)
I have been trying to design a plasmid for a helper dependent adenovirus but while looking i wasn't able to see a backbone specifically for HDAds so I was wondering if a general adenovirus backbone ...
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How to model the relationship between environments based on environmental covariates in a genomic prediction context
I have a dataset with different wheat lines in multiple environments. For each line various traits are measured (e.g. grain yield). I am trying to set up various genomic prediction models (linear ...
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Is it possible to improve metabolic rate through quorum sensing?
I am building a microbial fuel cell unit using mint plant and soil microbes. My goal is to power a LED light using the electricity but the electrify that microbes are so little so I am thinking of ...
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Method of determining base values of traits in isolated populations
Prelude: I came across a discussion about the correct formula for calculating the average IQ of offspring, which goes something like the following
$$ 100 + \frac35 \left( \left(\frac{\text{father's IQ ...
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How would you find out which gene is causing a specific phenotype in Drosophila through crosses only?
I'd recently attended an interview and was asked a question I've been trying to get to the bottom of. The question was that there is a mutation in which some smaller drosophila are only attracted to ...
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How to understand restriction mapping a quarter of a century later?
Today, biology is virtually all based on massively parallel sequencing, long-strand sequencing, and metagenomic; looking back at old restriction mapping is not straightforward (at least for me).
For ...
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Gene Mapping - Please explain in Lay terms
Taking an AP bio course, and for some reason I cannot comprehend the mechanism of how one determines the location of alleles on a chromosome. The math is simple and I can memorize it, but its bugging ...
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Mechanism of random assortment of chromosomes
How does the random assortment of chromosomes during meiosis occur?
I am a mathematician, not a biologist, and I am surprised that it is difficult to find an answer to this question online (AI ...
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How random is genetic recombination?
Two parents can have a very large number of different potential offsprings, and it's common knowledge that the daughter chromosomes in meiosis are produced randomly, i.e. in crossover, the exact spots ...
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About tomatoes pigments, why red tomatoes rather than yellow ones
How the first tomatoes (yellow) that arrived in Europe turned from yellow to red ?
I know the yellow pigment is xantophylle and the red one is lycopene, but my question is, was it an adaptation of the ...
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Genetic entropy [closed]
One day my internet acquaintance told me, that genetic entropy is evidence for intelligent design and he also provides me this waiting time problem and this simulating evolution by gene duplication.
I ...
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What has caused my oxeye daisies to mutate?
Some oxeye daisies (Leucanthemum vulgare) in my garden are showing strange mutations. I have a few oxeye daisy plants around my garden in different areas, but only one plant's flowers are showing ...
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Production method of custom seed mir451? How to edit middle of a fragment?
I am trying to create a plasmid that produces mir451 targeting a new gene, so I need to alter the seed sequence. The seed sequence is 7 bp long, so I think that is too long for PCR mutagenesis. How do ...
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