Questions tagged [bacteriophage]
Bacteriophage (phage) are viruses that infect bacteria, though some definitions include viruses that infect prokaryotes more broadly (bacteria and archaea). They are distinct from eukaryotic viruses in their morphology and evolutionary history.
18
questions
2
votes
0
answers
34
views
How to understand restriction mapping a quarter of a century later?
Today, biology is virtually all based on massively parallel sequencing, long-strand sequencing, and metagenomic; looking back at old restriction mapping is not straightforward (at least for me).
For ...
5
votes
1
answer
73
views
How was Campbell's Model of phage lambda integration into E. coli definitively proven?
In a series of seminal papers summarized in Annual Review in Genetics (2007) 41 1–11, Allan Campbell proposed a model that assumed phage lambda integration in its host (E. coli) genome. This is in ...
3
votes
1
answer
480
views
Who demonstrated that phage lamba is integrated into the host chromosome?
In the 1950s, the Lederbergs (Esther and Joshua) demonstrated the presence of phage lamba in Escherichia coli K-12.
But who demonstrated that this virus is integrated into the host's genome? Is there ...
1
vote
2
answers
55
views
Who discovered that phage phiX174 has a single-stranded genome?
I have recently read that Erwin Chargaff has discovered that the genome of phage phiX174 (ϕχ174) is single-stranded. However I could not find the paper reporting this discovery.
Is there a link to the ...
0
votes
1
answer
71
views
How did the Zinder-Lederberg experiment on Transduction work?
In the paper that introduced TRANSDUCTION (J Bacteriol. 1952 Nov;64(5):679-699), Lederberg and his student Zinder reported that S. typhimurium
"LT-22 is lysogenic for a virus active on LT-2. ...
0
votes
0
answers
21
views
Is there a way to isolate bacteria from a bacteria/phage plaque in order to PCR bacteria only?
I am writing a paper for a class that involves a mock grant proposal. I am looking at an experiment which requires sequencing of host DNA from a virus/host model, and am having a hard time finding a ...
0
votes
0
answers
20
views
How to calculate phage infection rate from decreased PFU counts?
Some experiments measured the effect of particular chemicals on the infectivity of lytic phages in terms of influence on the PFU/mL counts.
For example, the reduction of PFU/mL of exposed phages over ...
0
votes
1
answer
60
views
Is there a way to lyse bacteriophage with just heat?
I basically want the virus to fall apart into pieces. I want the capsid proteins to separate from the tail proteins and the DNA to all tumble out. I want all of the virus proteins, structural and DNA, ...
7
votes
1
answer
429
views
Has there been evidence that there has been coevolution in E. Coli strains with the T4 bacteriophage?
According to Evolution of T4-related phages, there have been multiple instances of evolution in the T4 coliphages. Per the article, there are many T-even phages that have insertions from other ...
2
votes
1
answer
79
views
Is there a standard protocol for isolating bacteriophages from biofilm?
We are planning to screen soil, water, and biofilm for mycobacteriophages, particularly the environments where the bacteria have been found in the past.
We have already isolated phages from soil but ...
5
votes
1
answer
845
views
Why does adding calcium chloride aid in bacteriophage infection?
We are attempting to isolate bacteriophages from environmental soil samples and most protocols recommend adding CaCl2 to the media to aid bacteriophage infection, even though they all recommend ...
2
votes
1
answer
100
views
Does centrifuging and filtering supernatant confer an advantage compared to just filtering?
We are trying to isolate bacteriophages from environmental soil samples. One of the first steps is elution, centrifugation and filtering of the soil samples.
One protocol suggests to:
Elute the soil ...
0
votes
2
answers
4k
views
Why do bacteriophages look like tiny little robots? [closed]
I get it, evolution is funny and works in mysterious ways. But come on. You can't tell me this thing doesn't look like a nanobot.
Why do bacteriophages look this way? With their cylindrical heads and ...
4
votes
1
answer
138
views
How can I change the payload of a bacteriophage used to transform E. coli?
I was looking at bacteriophages and how they're used to transform E.coli. While the whole process of how a bacteriophage works makes sense theoretically, I wanted to know how one goes about changing ...
3
votes
2
answers
71
views
How did plaques form in Salvador Luria's Bacteriophage experiment (discovery of restriction enzymes)?
I was watching Eric Lander's lecture online describing Luria's experiment (see below for link), and I couldn't understand how plaques form. From my understanding,
Bacteriophage infect strain B
Viral ...