All Questions
Tagged with genetics gene-expression
121
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What backbone to use for Helper dependent adenovirus (HDAd)
I have been trying to design a plasmid for a helper dependent adenovirus but while looking i wasn't able to see a backbone specifically for HDAds so I was wondering if a general adenovirus backbone ...
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44
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How exactly are the eye color genes related?
How exactly are the gey (maybe aka EYCL1?), bey(2?) (aka EYCL3?), HERC2, and OCA2 genes related to each other? There are numerous sources that explain eye color in terms of OCA2's role in the creation ...
- 111
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How does positional memory in fibroblast cells work?
I have a question regarding how positional memory in fibroblasts work, specifically, where the memory comes from/how its made.
For example, the Salamander & other amphibians are able to regenerate ...
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1
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What is a good expression vector for Salmonella enterica subsp. enterica serovar typhimurium
I am interested in expressing custom proteins in a Salmonella strain, however I am facing difficulties in finding the appropriate expression vector for it. It seems that most of the resources provide ...
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1
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How do DNA methylation canyons/DNA methylation valleys/DMCs differ from CpG islands?
Are these just both regions of the genome that are undermethylated? Is the only difference that for something to be a CpG islands it needs a high level of CpG sites and DMCs don't?
So could a 3.5kb ...
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Function of SMAR in plasmids?
A few years ago, the Thought Emporium published a video (https://www.youtube.com/watch?v=aoczYXJeMY4) in which he refers to a study in which they mix plasmid DNA with Chitosan and feed it to mice to ...
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Copy number deletion and high expression [duplicate]
I have a region that has significant CNV loss. But the expression for the genes in those regions is high compared to the expression found in the samples that don't have the regions lost. How can this ...
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242
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Are all genes capable of being switched on or off?
Are all genes capable of being switched on or off or only some genes? Are there some genes that permanently do not have the functionality that enables them to be switched on or off?
Everything I have ...
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How I can find the list of transcription factor proteins involved in transcription of a specific gene?
I want to get the list of transcription factor proteins involved in the transcription of the human SIRT1 gene. How can I access that?
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Monogenic disorders vs multifactorial inheritance disorders
There's a condition called SYNGAP1-related intellectual disability which is caused by mutations to the SYNGAP1 gene.
I believe that this is called a monogenic disorder, while disorders that are caused ...
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84
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How could I make my fish gold?
I'm doing research on how the pigmentation of fish. I want to know how could you change it with the help of genetic modification. I have some Silver Dollar fish (Metynnis argenteus) and I want to make ...
5
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1
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243
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Quantifying Gene Expression
I have found that many studies use the mRNA concentration as a “proxy” for protein activity because there should be correlation between mRNA levels and proteins expression levels. How is protein ...
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How do genes determine facial features?
What is known about the genes responsible for inherited facial features — the family resemblances that are so recognizable? Take for example a particular shape of nose: which gene or genes make it ...
user60511
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151
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Limits of gene editing
I was reading some articles about CRISPR and the world of gene editing, but then a lot of questions for which I couldn't find any answer online came into my mind. Those are all about how far can we ...
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Help Finding Specific BlaZ Gene Type Sequences on Genbank
I am doing an undergraduate research project that involves blaZ gene typing for different strain types of Staphylococcus aureus bacteria; for reference, here are some of papers on this topic that ...
