All Questions
Tagged with genetics molecular-biology
213
questions
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Why do we choose to use agar instead of agarose in various microbial applications?
When performing gel-electrophoresis we always use agarose. Is there a reason we can't perform it using Agar?
And in microbial culture Agar is commonly used as solidifying agent, could this be replaced ...
3
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2
answers
158
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Change of DNA concentration due to restriction digest?
Assume that you perform a restriction digest in a molecular biology lab: you combine genomic DNA, a restriction endonuclease (e.g., EcoRI), and the optimal buffer for that endonuclease and are about ...
3
votes
1
answer
135
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Can a person have different sex at cellular level?
I mean like every cell has a sex chromosome.So does a male with XY chromosomes has all the cells in all the organs inside his body of XY chromosomes only? And vice versa.....
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2
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198
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Why is the codon size three, rather than four? [duplicate]
The genetic code consists of triplets, each of which (apart from the stop codons) yields an amino acid when the mRNA is translated.
But why did triplets evolve, rather than a longer or shorter codon ...
- 29
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43
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ddT-tailed TA cloning, and the fate of a double nicked plasmid in E. coli
In an undergraduate lab class on TA cloning, it was explained that ddNTPs are used as the substrate for terminal transferase when making the T overhangs of the vector. I was told this was to ensure ...
- 113
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212
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Why is There a Necessity for Two Rounds of Cell Division and Four Daughter Cells in Meiosis
Why does meiosis involve two rounds of cell division instead of stopping after meiosis I, where each daughter cell would have one chromosome randomly selected from each pair of homologous chromosomes? ...
- 139
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89
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Is there a name for this type of diagram?
Is there any commonly used name for this type of diagram / symbology? I have not been able to find them referred to by any name.
- 151
3
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506
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Is there any disease that is proven to originate from malfunction of alternative splicing or post translation changes in the genome?
The most common diseases with genetic origin like muscular dystrophy seem to originate from issues with the primary structure of the DNA itself. Also it seems to be somewhat controversial as to ...
- 99
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213
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Molecular biology - Lewin's Gene Xi vs Molecular Biology of the Cell by bruce albert [closed]
I am an undergraduate and want to start reading about molecular biology. to anyone who has read both books please let me know your thoughts. which one should I go with?
Lewin's Gene Xi or Molecular ...
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3
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1
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304
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Function of SMAR in plasmids?
A few years ago, the Thought Emporium published a video (https://www.youtube.com/watch?v=aoczYXJeMY4) in which he refers to a study in which they mix plasmid DNA with Chitosan and feed it to mice to ...
2
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1
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43
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Receptors and their distribution over the human population
For humans it has been reported that there are three vasopressin receptors (AVPR1a) and four dopamine receptors (DRD2). (Source: UNIPROT)
Question: Does every human contain all three variants of ...
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113
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Is retinoblastoma truly an autosomal dominant condition?
I am getting, in my opinion, very conflicting information from sources about the inheritance of retinoblastoma, a type of cancer. Hereditary retinoblastoma is associated with defects in the gene ...
2
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0
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57
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Apparant inconsistency in DNA topology theory in formation of origin of replication [duplicate]
I'm studying an introductory course in genetics and came across something I don't fully understand. I obviously used Google to find where I'm thinking wrong, but I still can't understand it.
To ...
- 29
3
votes
1
answer
105
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How can SNP arrays be used to detect deletions within a gene?
I am reading a journal paper where the researchers are studying the effect of disease-causing mutations in the IL1RAPL1 gene. In the first figure of this paper, they show pedigrees of families where ...
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3
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Use of plasmid pXen5 for transposon screening
I would like to use the plasmid pXen5 (by Xenogen) for a transposon screen.
It contains two inverted repeat sequences, with Luciferase, Kanamycin, and the transposase itself in between. (It's tn1409).
...
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