Questions tagged [molecular-genetics]
The scientific study of the structure and function of genes at the molecular level, particularly chromosomes and DNA.
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How do I carry out an in silico PCR on a bacterial genome (target:16S rRNA)? Unable to find any bacterial genome on UCSC Genome Browser in silico PCR
I could not find any bacterial species in the drop-down list for genomes in https://genome.ucsc.edu/cgi-bin/hgPcr
Any kind of help would be appreciated. Other links for in silico PCR did not really ...
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What backbone to use for Helper dependent adenovirus (HDAd)
I have been trying to design a plasmid for a helper dependent adenovirus but while looking i wasn't able to see a backbone specifically for HDAds so I was wondering if a general adenovirus backbone ...
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Role of Automata Theory in Biology
Lately I have been taking an interest in mathematical biology. While reading "Evolutionary dynamics" by Martin Nowak, I came across this reference, where the author describes in short how ...
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Do cells know or imagine how each part of the DNA influences body structure and function? How else would evolution happen in specific ways?
Consider how an octopus looks at a rock and it's entire body blends in to resemble the rock. Consider how insects evolved to resemble leaves or twigs. Even frogs, butterflies and other creatures ...
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Can the mechanism behind changes in protein expression be inferred by the timing of those changes?
My question relates to the timing of protein expression changes, and whether there is a literature-based consensus as to how long changes in protein generally take.
For example, if protein changes 24h ...
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Understanding DNA Fingerprinting
I am a high-school student learning about DNA fingerprinting. I know that satellite DNA is non-coding DNA and has a lot of repetitive sequences, and the length of each repeat can be either short or ...
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Is Regenerative Brain Tissue Technology In The Near Future?
I got 99% of my information from articles I found in NIH's library; I can't cite anything now because I am on a timed session in a public library.
I've been reading material from research papers in ...
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How do you find the template strand for a sequence where start codon is in both strands?
Maybe I'm missing something very obvious but I can't seem to understand how to find the template strand given a sequence and its complementary strand if both strands contain ATG from 5' -> 3'. ...
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Preserving a sample for mtDNA and nuclear DNA analysis
Imagine a person in the early half of the 20th century (1900 to 1950) took some kind of sample(s) from a living human body using any technology of the era. They then stored it using any technology ...
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With CRISPR-mediated gene editing, can the PAM for the guide RNA also be site of the desired edit?
I'm hoping to use CRISPR-Cas9 in yeast to change a single nucleotide in a gene of interest. While there are a couple potential guide RNA sequences I could use, the best option's PAM sequence (TGG) it ...
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Why is the expected time to coalesce the same as the ploidy times inbreeding effective population size?
The expected time to coalesce, in generations, is the same as the ploidy (e.g., 2 for humans) times Nef, the inbreeding effective population size, under coalescent
theory. Why?
Both ploidy * Nef and ...
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Have there ever been "half-twins" who were halfway between siblings and twins?
Have there ever been two human siblings who were 75% or more genetically related?
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Has perturbation theory been applied to mutation process frameworks?
For example, imagine this Feynman diagram:
This is analogous to mutational homoplasy.
When comparing haplotypes, there are many possible tree topologies. Under maximum parsimony, we ignore suboptimal ...
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Sequencing a PCR product with no band
I did a PCR that successfully showed the appropriate bands one time, but when I tried to re-do the same PCR just at a larger quantity to send for Sanger sequencing, very faint or no bands were seen. ...
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How come SSBPs in RPA don't bind primers?
I've started reading about the recombinase polymerase amplification (RPA). I'm learning that in RPA, recombinase enzyme binds to primers, then makes them anneal to the complementary target DNA strand, ...
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