Questions tagged [mutations]
A change in an organism's genomic sequence.
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Has anyone tried site-directed mutagenesis aimed at deleting >10 amino acids of a protein using overlapping primers?
I am trying to use primer pairs with overlapping and non-overlapping regions to create deletions within a protein sequence (https://bmcbiotechnol.biomedcentral.com/articles/10.1186/1472-6750-8-91).
I ...
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What is the probability distribution of a neutral mutation's allele frequency after k-generations? (in asexually reproducing organisms)
I've had trouble finding info on how to model this problem, so figured I'd ask it here. I'm trying to figure out what the probability distribution is of the frequency of a mutation in a population ...
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What has caused my oxeye daisies to mutate?
Some oxeye daisies (Leucanthemum vulgare) in my garden are showing strange mutations. I have a few oxeye daisy plants around my garden in different areas, but only one plant's flowers are showing ...
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DNA slippage as the cause of insertion mutations in cancer cells?
I'm a computer science student who has started working with DNA. I know the basics but not everything.
While working on the ICGC data, I found a weird pattern in the insertions:
In around 60% of the ...
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Best way to predict the effects of deletion mutations on protein function?
I have the coding sequences of a WT gene and several mutants of this gene (deletion mutations varying from 5bp to 50% of the sequence deleted). What is the best method for inferring the impact of ...
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Has perturbation theory been applied to mutation process frameworks?
For example, imagine this Feynman diagram:
This is analogous to mutational homoplasy.
When comparing haplotypes, there are many possible tree topologies. Under maximum parsimony, we ignore suboptimal ...
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How did the fusion of Chromosome 2 spawn a separate smarter species?
I’m familiar with the evidence that two primate chromosomes fused into human Chromosome 2, and I understand that primates with such a mutation could still breed with those without it.
My question is, ...
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What causes the activation of "Late acting deleterious genes" in late age but not in young age, whose accumulation causes ageing according to Medawar?
Sir Peter Medawar proposed that aging is the byproduct of "late acting deleterious genes". Evolution is good at weeding out genetic mutations that are harmful at an young age, before the ...
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Is there potential to modify GM crops to inhibit their reproduction with non-GM crops?
I've been reading on terminator gene sequences and was wondering whether the same technology could be applied to GM crops to prevent transgene flow. Turns out Monsanto had developed the technology but ...
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Can co-mutations be potential expression quantitative trait loci (eQTLs)?
I'm wondering if it is possible to test this and how? Usually we do this for single SNPs, I wanted to know if it was plausible for pairs of SNPs. Which method would you suggest?
I want to test whether ...
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Is crossveinless in Drosophila melanogaster example of condition mutant or phenocarpy?
This is the original question -
Ques - A researcher exposed Drosophila larvae to 37°C during their growth. One of the adult flies that emerged had a crossveinless phenotype. When this crossveinless ...
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Ames Assay Confusion: Aren't the odds of spontaneous revertants too low to be able to accurately test the mutagenicity of certain compounds?
I am a student conducting a test with the Ames Assay. This assay uses a strain of bacteria that has a mutation in an amino acid synthesizing operon, which doesn't allow it to synthesize its protein. ...
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Can someone with albinism have red hair with very pale skin and blue eyes?
I have heard of a type of albinism that occurs in races with darker skin that can give people red hair, but in the pictures I've seen, they usually have reddish-brown hair, a medium skin tone, and ...
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Are mutation rates normally distributed? If not, what are they?
On average, there are 64 mutations per generation in the human genome. Is this constant, or can we expect variation in the number of mutations?
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Is there an example in evolution where a huge jump in evolution happened due to a dramatic mutation?
Here is a chicken that due to a mutation got 4 legs:
I wonder, are there examples, where such one-time dramatic mutational chages gave rise to a new species?
Are there species that appeared not due ...
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