Questions tagged [bacteriology]
A subdivision of microbiology dedicated to the study of bacteria.
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Post-Translational Modification in insulin production
When searching "How is insulin produced commercially" on Google, most results simply say something along the lines of "The insulin gene is inserted into a bacteria, which then express ...
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Why is the assimilation of single carbon sources exclusively, rather than other elements, used for phenotypic identification of bacteria?
Background
Single element source assimilation testing:
determines if an organism can grow using a single source of the specific element being tested. These tests constitute many of the tests in the ...
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What is (are) the species responsible for Lake Natron red waters?
Lake Natron is an alkaline lake located in northern Tanzania. It is known for its red waters, as seen on this image:
Natural-color Landsat 8 image showing Lake Natron in March 2017. NASA, public ...
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Metabolism of Purple non-Sulfur bacteria
I am a high school student who is currently experimenting with hydrogen sulfide absorption using bacteria. I know species of purple sulfur bacteria or green sulfur bacteria are able to oxidize ...
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Can saltwater bacteria grow in petri-dish using the same agar nutrient media?
I am planning on growing bacteria from a seawater sample to do a research project on the relationship between pH (lowered using sulfuric acid) and the bacterial concentration of the water sample (...
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How do I grow 5 liters of purple non-sulfur bacteria?
I am running experiments for a project that requires large amounts of purple non-sulfur, Cyanobacteria and nitrogen fixing bacteria. I plan on growing all the species in separate tanks. The species' I ...
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What is a reference strain in DNA-DNA hybridization DNA groups?
Results
DNA groups identified:
All intra-group relatedness values are shown in
Table I.
By means of reference strains, most of the DNA groups could be identified as groups described by Bouvet & ...
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Where does Actino- come from in the genus Actinomycetota?
This comment under Bacteria trapped in crystal inclusions found 'alive' after 50,000 years - what were they eating all that time? links to Quintana, Badillo ann Maldonado (2013) ...
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What is the largest culturable bacterium?
E. coli is easy to grow in the lab. It has a volume around $0.6 \mu m^3$. Source
T. namibiensis can be much larger, with a volume on the order of ${10^6}\mu m^3$. Source
However, T. namibiensis is not ...
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Does heterogeneous resistance have an impact on the zone diameter observed in disk diffusion susceptibility testing?
Question
Does the presence of small, discrete colonies within the zone of inhibition, suggesting heterogeneous resistance, impact the zone diameter in disk diffusion susceptibility testing?
"Help ...
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Can bacterial DNA solutions, which include other cellular components, be stored without compromising DNA stability?"
Question
Can bacterial DNA solutions, which include other cellular components, be stored without compromising DNA stability?"
Details
I'm using the boiling extraction method to extract ...
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How is the zone of inhibition formed in disk diffusion?
Question
How is the zone of inhibition formed in disk diffusion?
What I mean by the formation of the zone of inhibition is not the ultimate visibility of such zone but the circumstances that result in ...
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What's pooling in the spread plate technique?
"Distributing the organisms by rotating the spreader rather than the plate tends to cause more pooling of the inoculum."
https://asm.org/ASM/media/Protocol-Images/Preparing-Spread-Plates-...
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Does inoculum volume affect spread plate outcome?
Background
The spread plate method is a technique used in microbiology to enumerate and isolate bacteria or other microorganisms present in a sample. It involves evenly spreading a liquid sample ...
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Did operon evolve after endosymbiosis and specialisation of mitochondria?
We know that all extant bacteria use the operon system as their mode of regulating gene expression, in contrast to eukaryotes, which use individual gene promoters as well as epigenetic mechanisms, ...
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