Questions tagged [fluorescence]
For questions relating to the radiation emitted by substances as a result of incident radiation.
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Fluorescence Intensity and Fluorescence Quantum Yield
I plan to use ImageJ to analyze some fluorescence experiments where the pH changes. I will be using fluorescein as the indicator for the pH changes and it will be present in low concentration (like is ...
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How ph affects aesculune fluorescence?
I am facing a ween problem. I am trying to find out, how would look like the graph for fluroscence of aesculine (intensity of fluoroscence on Y axis and pH on X axis).
I know, that increasing the pH ...
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Microcopy Fluorescence filter set usefulness
I have a brief doubt and maybe someone around here can give as some help. We are doing a pilot study on microplastics using a fluorescence method (red nile). The dye is solvachromic, so it's emission ...
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Fluorescence quenching by palladium(II) vs. platinum(II) complexes
Why do we observe much stronger (in most cases complete) fluorescence quenching by $\ce{Pd^2+}$ than $\ce{Pt^2+}?$ I work with complexes of both metals and there is no real 100% explanation I can find ...
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Why is there a mirror image effect in the absorbance and fluorescence spectra?
I've read some answers online but I still can't seem to understand the mirror image effect. Why is the highest energy absorption (v" = 0 to v' = 4) the lowest energy fluorescence (v' = 4 to v&...
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Which commonly available UV light source to use with fluorescein
I want to start experimenting with fluorescein. I have a 353 nm UV light source, but am thinking that it might be too short since it looks like peak excitation occurs at 494 nm. So am I correct in ...
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Why do different substances containing saturated hydrocarbons burns with different flame?
LPG and Candle wax both contain saturated hydrocarbon, yet LPG gives a clean white flame but a candle gives yellow flame with lots of smoke. Why so?
I have read some answers on web which says that it ...
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Help with Forster distance conversion for FRET
For my project on Fluorescence, I'm trying to simplify the equation for the Forster distance (I obtained this from the Lakowicz book). To keep track, the units are in square brackets.
$$R_0 = \left(\...
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Why does the emission of dansyl group diminish with time in this experiment?
I don't know how to make sense of this. I what is happening when an enzyme (carboxypeptidase, which contains tryptophan as its only chromophore and uses a Zn(II) ion in its active center) hydrolises a ...
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Fluorometer for measuring fluorescence in a sheet of water
Does anyone know of a fluorometer that I could use to measure fluorescence in a thin sheet of water. So it would be like holding a piece of writing or printer paper vertically and trying to get a ...
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Implications of the excitation spectra
The fluorescence excitation spectra show the change in fluorescence intensity as a function of the wavelength of the excitation light.
I'm interested in the certain physical implications of the above.
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Absorption and emission at same wavelength?
Is it possible for a molecule to absorb and emit at the same wavelength? What is the reason behind it?
I’m working on charged tin porphyrins and got the excitation and emission (fluorescence) ...
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Data analysis in TCSPC for fluorescene decay (reconvolution with measured IRF)
I am trying to understand the data analysis of fluorescence decay counts measured by TCSPC technique, particularly with reconvolution with measured IRF.
I am able to get the fitted counts (given by ...
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Chemical Potential in the Fluorescent Molecule
I'm reading this paper, which states that the chemical potential $\mu$ is determined by the steady-state balance of up and down transitions in a fluorescent molecule. I am happy with this ...
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Common chemcials that produce phosphorescence
Are there any common lab chemicals or household stuff that produce phosphorescence?
I am aware of ZnS, CaS, Fluorescein, Erythrosin B, Vanillin Benzaldehyde, Vitamin B2, Tryptophan etc.
I just need ...
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