All Questions
Tagged with fluorescence spectrophotometry
11
questions
6
votes
1
answer
264
views
Lifetime components in phosphorescence decay
I'm studying the phosphorescence decay of diacetyl in acetonitrile (it has a high quantum yield of intersystem crossing). The fluorimeter than I'm employing has microsecond resolution and, since the ...
user78790
6
votes
2
answers
1k
views
How does molecular structure contribute in the property of fluorescence?
Fluorescence is the property which is exhibited when electrons emit wavelength of light lower than the one they absorbed so does that mean we can make every molecule to become fluorescent? I wanted to ...
1
vote
0
answers
45
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How can I save an EEM file on a Fluoromax4?
I'm trying to run excitation-emission matrices on some water samples, but I'm running into some software issues that the manuals aren't helpful for. In order to use R studio to extract my data, I need ...
- 61
2
votes
0
answers
1k
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Data analysis in TCSPC for fluorescene decay (reconvolution with measured IRF)
I am trying to understand the data analysis of fluorescence decay counts measured by TCSPC technique, particularly with reconvolution with measured IRF.
I am able to get the fitted counts (given by ...
- 139
1
vote
0
answers
102
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Common chemcials that produce phosphorescence
Are there any common lab chemicals or household stuff that produce phosphorescence?
I am aware of ZnS, CaS, Fluorescein, Erythrosin B, Vanillin Benzaldehyde, Vitamin B2, Tryptophan etc.
I just need ...
- 139
1
vote
0
answers
458
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Choosing the right wavelength for calibration curve and for analysis of actual results
Introduction:
I am currently conducting a series of experiments in which I am studying the transport of polystyrene nanoparticles (PNPs) through sand and soil. This is done in sand/soil columns, into ...
- 1,410
2
votes
1
answer
2k
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Is Beer-Lambert law also valid for fluorescence and luminescence?
When I measure optical density in a microplate reader the absorbance is proportional to the concentration and the height of the liquid. Is Beer-Lambert law also valid for fluorescence and luminescence?...
- 123
14
votes
1
answer
612
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Are there any (simple) molecules with very different absorption and emission dipole directions?
When a single molecule absorbs or emits light, it does so perpendicular to the direction of the respective transition dipole. In principle, the directions of the dipoles for absorption and emission ...
- 275
1
vote
2
answers
129
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Is this online fluorophore database reliable?
I need fluorescence spectra for various fluorophores in certain dispersion media for some calculations. There is an online database of University of Arizona: http://www.spectra.arizona.edu/
Is using ...
- 11
3
votes
1
answer
593
views
Why do fluorescence intensities decline with replicate number?
I am performing a determination of riboflavin with fluorescence spectrophotometry. I've dissolved my riboflavin in a dilute GAA solution, created a calibration curve and measured my unknown's ...
- 2,354
1
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2
answers
2k
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What makes a species "fluorescence quencher"?
I just came across an article where they said that sodium fluoride is not quencher (in the context of fluorescence). Why is NaF not a quencher but NaBr is one?
user14874