All Questions
Tagged with fluorescence spectroscopy
12
questions
5
votes
3
answers
352
views
Excited states and emission lifetimes
I have some doubts about the nomenclature of the lifetimes. Are singlet and fluorescence lifetimes the same? As well as the triplet and phosphorescence lifetimes?
Also, can the triplet/phosphorescence ...
user78790
1
vote
0
answers
82
views
Spectroscopic methods for quantifying peptides/proteins with or without Tryptophan or Tyrosine content
I have several peptides (20-50 amino acids long) which I want to quantify the solubility/concentration in a solvent at certain temperature and pH.
These peptides may or may not contain Tryptophan or ...
- 189
1
vote
1
answer
162
views
Why does isolated chlorophyl emits red light?
If we observe isolated chlorophyl solution with UV light, chlorophyl is seen as red. Explanation suggests that when a specific atom of chlorophyl absorbs UV light, the atom gets excited and in a short ...
3
votes
0
answers
99
views
How can I set up and process my excitation-emission matrices properly?
I'm running EEMS on some water samples on a Horiba Fluoromax 4 spectrofluorometer. Method below.
$\pu{240-580nm}$ emission, $\pu{5nm}$ interval, $\pu{1nm}$ width.
$\pu{240-480nm}$ excitation, $\pu{...
- 61
1
vote
2
answers
475
views
Implications of the excitation spectra
The fluorescence excitation spectra show the change in fluorescence intensity as a function of the wavelength of the excitation light.
I'm interested in the certain physical implications of the above.
...
- 153
4
votes
1
answer
2k
views
Absorption and emission at same wavelength?
Is it possible for a molecule to absorb and emit at the same wavelength? What is the reason behind it?
I’m working on charged tin porphyrins and got the excitation and emission (fluorescence) ...
- 49
2
votes
1
answer
576
views
Is the XRF spectroscopy equally sensitive for every element?
I am curious whether or not every element can be equally well detected by using X-Ray-fluorescence-spectroscopy. Might there be any contitions or circumstances that make it hard/impossible to detect a ...
- 105
0
votes
0
answers
73
views
Does something like a fluorescent foil or a fluorescent film exist?
During my experiments I want the emitted laser beams to come back with a different wavelength than the original wavelength using a fluorescent foil or fluorescent film.
The laser I'm currently using ...
- 9
1
vote
1
answer
1k
views
What is the relationship between emission and fluorescence?
A molecule exposed to a photon with some energy is put into an excited state, and emits a photon of some energy when it returns to ground state.
The photon that provided the energy for excitation ...
- 253
1
vote
0
answers
39
views
Does the laser side of laser fluorescence spectroscopy have to be so complicated?
I understand the working principle behind zapping some gas and looking at spectral emissions, and I can see why photodetection is so difficult. But I thought that pointing a laser into a vacuum ...
- 111
1
vote
3
answers
1k
views
What are the requirements for FRET?
I have a question about fluorescence resonance energy transfer (FRET), and the requirements needed for this technique.
Q: Which of the following need to be met for FRET?
A) Strong overlap between ...
- 13
1
vote
2
answers
2k
views
What makes a species "fluorescence quencher"?
I just came across an article where they said that sodium fluoride is not quencher (in the context of fluorescence). Why is NaF not a quencher but NaBr is one?
user14874