All Questions
Tagged with fluorescence analytical-chemistry
9
questions
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Why isn't residual standard deviation used in FQY analysis?
I've been measuring relative fluorescence quantum yield (hereafter FQY) for a photosensitive pigment. Most analytical articles and standard operating procedures (SOPs) I've read suggest taking the ...
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3
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Wave Dispersive X-ray Fluoresence and Bragg's Law
Thermo Fisher website explains that "WDXRF uses crystals to disperse the fluorescence spectrum into individual wavelengths of each element, providing high resolution and low background spectra ...
user119534
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71
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Why wouldn't a standard addition fluorometric method return the same starting concentration of a solution used to make the standards?
I am trying out a new standard addition method and having some very basic problems. I am hoping someone could point me in the right direction.
I am using a fluorometric method to quantify ammonium in ...
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1
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1
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Latent blood detection with fluorescein
I need help with the reaction between fluorescein and blood in the method of detecting latent blood stains.
I find it hard to find any literature on this subject.
As far as I am concerned, the ...
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When an electron is excited to a singlet state, must it flip its spin state to enter the triplet state and flip once more to the ground state?
I understand the Jablonski diagram in that it has intersystem crossing from the singlet state to the triplet state, but how many spin switches are necessary to complete to phosphores? When an electron ...
- 21
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458
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Choosing the right wavelength for calibration curve and for analysis of actual results
Introduction:
I am currently conducting a series of experiments in which I am studying the transport of polystyrene nanoparticles (PNPs) through sand and soil. This is done in sand/soil columns, into ...
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2
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1
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Is Beer-Lambert law also valid for fluorescence and luminescence?
When I measure optical density in a microplate reader the absorbance is proportional to the concentration and the height of the liquid. Is Beer-Lambert law also valid for fluorescence and luminescence?...
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Calcium detection using XRF
I have a mixture of metal oxides in powder form. Mainly Iron oxide, silicon dioxide, and calcium oxide. I know for certain calcium oxide is in the mixture, but my handheld XRF is not detecting it.
I ...
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3
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Why do fluorescence intensities decline with replicate number?
I am performing a determination of riboflavin with fluorescence spectrophotometry. I've dissolved my riboflavin in a dilute GAA solution, created a calibration curve and measured my unknown's ...
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