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3 votes
0 answers
38 views

Detection of contamination from salicylic acid via either single excitation fluorometer or via absorbance

My colleague detected fluorescent contamination likely from salicylic acid in some water samples. I have made a new batch and want to check whether my new samples also have this contamination. 1. Can ...
claire's user avatar
  • 31
0 votes
0 answers
465 views

Why is there a mirror image effect in the absorbance and fluorescence spectra?

I've read some answers online but I still can't seem to understand the mirror image effect. Why is the highest energy absorption (v" = 0 to v' = 4) the lowest energy fluorescence (v' = 4 to v&...
Mark's user avatar
  • 31
4 votes
1 answer
2k views

Absorption and emission at same wavelength?

Is it possible for a molecule to absorb and emit at the same wavelength? What is the reason behind it? I’m working on charged tin porphyrins and got the excitation and emission (fluorescence) ...
Pavithra J's user avatar
2 votes
2 answers
1k views

Why do some molecules show non-symmetric absorption and emission bands?

I'm looking at a molecule of [Ru(bpy)3]2+ in water and the absorption and emission bands are not symmetrical. I want to know why the mirror image rule doesn't apply here. Is this because of the MLCT, ...
Abu's user avatar
  • 23
1 vote
1 answer
1k views

What is the relationship between emission and fluorescence?

A molecule exposed to a photon with some energy is put into an excited state, and emits a photon of some energy when it returns to ground state. The photon that provided the energy for excitation ...
P...'s user avatar
  • 253