Questions tagged [buffer]
Use for questions regarding solutions containing a mixture of a strong acid and weak base or vice versa.
79
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Non-binding buffer at pKa of roughly 6 that dissolves in ethanol?
I'm trying to find a proper buffer for my experiment, which I'd appreciate some suggestions on. I have a solution in which I want to measure the concentration of magnesium ions using a fluorescent ...
- 31
-1
votes
1
answer
79
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Calculating the pKA value of a mixture of two weak acids?
Will it be possible to find the pKa value of two weak acids? I am working on a project for school on calculating buffer capacities and to find the pH of the buffer solution, I have seen that you need ...
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-2
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1
answer
49
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Calculate the buffer capacity of a mixture/solution?
I am planning an experiment where I am measuring the buffer capacity of a mixture. However the mixture has two weak acids in it. Will having multiple weak acids in the mixture cause any changes in the ...
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1
vote
1
answer
33
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Dihydrogenphosphate and hydrogenphosphate buffer solution
I have just come across a problem involving a buffer solution comprising of dihydrogenphosphate ions and hydrogen phosphate ions, which asks how the mixture of these two ions can function as a buffer ...
3
votes
0
answers
35
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Assessing stability of protein used for interaction experiments after changing buffer from tris to HEPES
I have a protein with a theoretical $\mathrm{pI}~9.0$ currently stored in $\pu{25 mM}$ tris and $\pu{250 mM}$ $\ce{NaCl}$ $(\mathrm{pH}~8)$ at $\pu{−80 °C}.$ I need to do experiments in HEPES at $\...
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2
votes
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63
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Stability of n-Dodecyl-beta-D-maltoside in buffer solutions for assay experiments
I have a buffer system which contains multiple salts and in addition DTT and n-Dodecyl-beta-D-maltoside.
I understand that DTT is hydrolysing quite fast and this component must be used always fresh ...
- 779
-2
votes
3
answers
333
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Why are buffer solutions not neutral
I am very confused about buffer solutions and I have lots of ideas about them which don’t integrate together so I really can’t tell which are correct and which are wrong. That being the case it’s ...
2
votes
1
answer
234
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Effects when 100 mmol NaHCO3 are mixed with 50 mmol HCl
I read somewhere that when $\pu{100 mmol}\ \ce{NaHCO3}$ are mixed with $\pu{50 mmol}\ \ce{HCl}$, a buffer arises. I'm confused, since $\ce{HCl}$ is a strong acid, and I thought the classic buffer is ...
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2
votes
0
answers
86
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Why do buffer solution only maintain pH to a certain limit?
I know that buffers have a capacity after which they are not able to maintain a relatively stable pH. This capacity is exceeded when we add too much acid or base but I was wondering why does this ...
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2
votes
2
answers
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Calculating the pH after adding hydrochloric acid to the potassium hydrogen phosphate solution
I am trying to calculate the $\mathrm{pH}$ of a solution obtained by mixing $\pu{20.00 mL}$ of $\pu{0.2 M}$ potassium hydrogen phosphate solution and $\pu{10.00 mL}$ of $\pu{0.25 M}$ hydrochloric acid ...
- 79
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What happens to the ions after dissociation in an aqueous solution? [closed]
I'm in high school studying about Buffer Solutions. We had an example of a solution made up of Ammonium Hydroxide and Ammonium Chloride for a Basic Buffer.
NH4OH ⇌ NH4 (+) + OH(-)
NH4Cl ⇌ NH4 (+) + ...
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-2
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1
answer
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Calculate the pH of a buffered solution after adding strong acid [closed]
Calculate the pH of a solution formed by the addition of $0.0020$ moles of $\ce{HCl}$ to $\pu{100.0 mL}$ of $\pu{0.200 M}$ $\ce{CH3NH2}$/ $\ce{0.100 M CH3NH3Cl}$.
$K_\mathrm{b}(\ce{CH3NH2})=4.4\times ...
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votes
1
answer
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Sodium Citrate / Sodium Phosphate Buffer Calculations
I'm trying to work through the calculations of a specific paper (linked here / below) but, my numbers are way off and I can't get them to match for the life of me. I need to make the same buffer but ...
4
votes
1
answer
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What is the math behind the formula for the concentration of the strong titrant when calculating theoretical buffer capacity of a diprotic system?
I have a question related to finding the theoretical buffer capacity of a diprotic system, specifically about the formula for the concentration of the strong titrant. In this post:
What is the formula ...
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0
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Exact calculation of pH of diprotic acid plus strong base
Another post has an excellent "exact" calculation for the [H+] (or pH) of a generic diprotic acid dissociating in water.
Combining acid dissociation constants to determine pH of diprotic ...
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