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Questions tagged [fluorescence]

For questions relating to the radiation emitted by substances as a result of incident radiation.

1 vote
3 answers
1k views

What are the requirements for FRET?

I have a question about fluorescence resonance energy transfer (FRET), and the requirements needed for this technique. Q: Which of the following need to be met for FRET? A) Strong overlap between ...
Ken Aizu's user avatar
6 votes
2 answers
1k views

What is special about the molecule luciferin that it can emit light?

A classic example of bioluminescence; the protein luciferin is oxidized by the enzyme luciferase, releasing energy as light. What is it that gives luciferin this ability? Why can't just about any ...
Marijn 's user avatar
  • 515
1 vote
2 answers
106 views

Is there a chemical which fluoresces only after coming in contact with water? [closed]

I require an easily available chemical compound which in its pure form, does not fluoresce under UV light. However upon coming in contact with water (at room temperature) it fluoresces under UV light....
bit_junky's user avatar
3 votes
1 answer
593 views

Why do fluorescence intensities decline with replicate number?

I am performing a determination of riboflavin with fluorescence spectrophotometry. I've dissolved my riboflavin in a dilute GAA solution, created a calibration curve and measured my unknown's ...
gannex's user avatar
  • 2,354
1 vote
1 answer
131 views

Thermochromic, fluorescent fluid

I'm a total layman in chemistry and am working on an interactive sculpture project and therefore am researching into fluids that have certain properties - long story short: we are thinking on having a ...
riccardolardi's user avatar
5 votes
1 answer
172 views

Compound with a bright green emission when electrically excited

I rolled back the edit because I really am looking for the following: I am looking for an element or compound that would have a bright green emission when electrically excited and is a gas at between ...
user103218's user avatar
1 vote
2 answers
2k views

What makes a species "fluorescence quencher"?

I just came across an article where they said that sodium fluoride is not quencher (in the context of fluorescence). Why is NaF not a quencher but NaBr is one?
user avatar
12 votes
2 answers
7k views

What should the Rehm-Weller equation look like?

The Rehm-Weller equation, which defines the energy change for photoinduced electron transfer according to Lakowicz "Principles of Fluorescence Spectroscopy" [1, p. 337] should look like: $$\...
saldenisov's user avatar
7 votes
1 answer
167 views

Interpretation of Figure - biofunctionalization

In this paper on page 1985 they say: Fluorescence analysis confirmed that enhanced protein binding at higher electron dose depends only to a small extent on the slight increase in the lateral ...
user1818's user avatar

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