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Questions tagged [chromatography]

collective term for a set of laboratory techniques for the separation of mixtures

7 votes
1 answer
2k views

What is the behavior of a Zwitterion during ion exchange chromatography?

We're interested in purifying a protein that has an azide moiety using ion exchange chromatography. Due to its unique structure, the moiety is a Zwitterion with a novel chemical behavior. My question ...
bobthejoe's user avatar
  • 2,400
10 votes
2 answers
9k views

Gas chromatography/mass spectrometry: Can I use peak ratios and molecular mass to derive quantity?

Can derive the quantity of a substance from a GC/MS report if I know: the ratio to another substance in the data, the quantity of that second substance, and the molecular mass of both substances. ...
Anthony's user avatar
  • 209

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