All Questions
Tagged with chromatography solvents
8
questions
5
votes
1
answer
404
views
How to effectively replace DMSO with LC–MS-compatible solvent for the samples from a well plate?
I want to do LC–MS analysis of the samples from a 384-well plate where each compound (at around 1–10 μM) is dissolved in 10–15 μL of DMSO. I am worried about the DMSO dwarfing the analyte’s signal, as ...
- 441
2
votes
0
answers
148
views
How to choose the solvent gradient for the flash column chromatography of a complex mixture?
When performing a flash column chromatography, it is advised to select an eluent in which the desired compound has an $R_\mathrm{F}$ of about $0.25$ in TLC.
However, if I want to separate and isolate ...
user78790
3
votes
0
answers
121
views
Why does column chromatography not work like it's supposed to?
I'm a few months into working in an organic synthesis lab and I consider myself to have a solid intuition for, well, how chemistry works. I have ran at least 50 columns at this point but no matter ...
- 31
1
vote
1
answer
70
views
Decomposable solvent identification in GC-FID
How can a gas chromatography with flame ionization detection (GC-FID) be used to identify a liquid solvent that decomposes at its boiling point like DMSO? In a solution with other higher boiling ...
- 127
1
vote
2
answers
182
views
Optimal lipophilic solvent for washing PEEK-lined reversed-phase HPLC columns
What would be the reasonable strategy for washing a reversed-phase (RP) octadecylsilyl (ODS) C18 HPLC columns from lipids and other non-polar residues? The columns are used for biochemical analysis of ...
- 38.4k
4
votes
1
answer
403
views
How to recover C18 column after prolonged exposure to phosphate buffer?
A colleague of mine who occasionally does HPLC in a biochemistry lab discovered that a C18 column was not properly flushed after the previous experiment.
It appears that the column was stored for ...
- 38.4k
3
votes
2
answers
362
views
Organic solvent for cleaning autosampler syringe
I work in a laboratory where gas chromatography is used extensively. We handle phthalates. What are the possible wash solvent for autosampler syringe? I've been using acetone.
- 31
2
votes
0
answers
573
views
Which are the appropriate washing solvents for gas chromatography equipment? [closed]
I work in a laboratory where gas chromatography is used extensively. We handle terpenes and terpenoids (alcohols, ketones, and acetates derived from terpenes), organic solvents (acetone, acetonitrile, ...
