All Questions
Tagged with chromatography terminology
3
questions
3
votes
1
answer
4k
views
Why is Rf called Retardation Factor?
I understand that $$R_\mathrm{f} = \frac{\text{distance traveled by center of analyte spot } (b)}{\text{ distance travelled by solvent front } (a)}$$ What I do not understand is why this is called ...
- 33
5
votes
2
answers
46k
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Normal phase vs reverse phase HPLC
What is the difference between normal phase and reverse phase high-performance liquid chromatography?
- 59
0
votes
1
answer
113
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The proteins "eluted" or "were eluted"?
In describing proteins, I've written "the proteins detached from the [Ni-IDA] matrix and were eluted from the column"
Should I use "were" or can something simply "elute" ?
- 672