All Questions
Tagged with chromatography reference-request
3
questions
5
votes
1
answer
2k
views
Source for UV cut-off values of buffers
When I want to check a $\mathrm{p}K_\mathrm{a}$ value I look it up in CRC Handbook. Is there such source for UV cut-off values of various buffers?
Usually books in chromatography contain tables with ...
- 51
3
votes
1
answer
133
views
Is there a list of must-have columns for LC-MS/MS used in biochemistry/clinical lab setting?
Considering a wide variety of chromatographic columns used for (U)HPLC based on
phase type (normal or reverse);
column dimensions (geometry, length, inner diameter);
particle material $(\ce{SiO2},$ $\...
- 38.4k
4
votes
1
answer
403
views
How to recover C18 column after prolonged exposure to phosphate buffer?
A colleague of mine who occasionally does HPLC in a biochemistry lab discovered that a C18 column was not properly flushed after the previous experiment.
It appears that the column was stored for ...
- 38.4k