All Questions
Tagged with chromatography biochemistry
10
questions
1
vote
2
answers
288
views
How does industry separate glucose from fructose using liquid chromatography when producing high-fructose corn syrup?
Can someone explain how the separation of glucose and fructose is achieved using liquid chromatography in the industrial production of high-fructose corn syrup?
I've seen references to ion-exchange ...
- 121
1
vote
0
answers
47
views
How to change the counterion of a nucleotide salt?
I need to take the sodium nucleotide salts I have available in my lab and modify them to instead contain some more exotic counterions. Can I do this by binding my nucleotides to a strong anion ...
- 137
2
votes
0
answers
106
views
Right conductivity of protein sample for Protein A purification of human IgG?
A sample from a cell culture has some IgG and this is to be purified using protein A chromatography. Based on the chromatography handbook from GE-Healthcare/Cytiva, the protein sample should have &...
- 119
3
votes
1
answer
138
views
Why can DAP be used to elute a protein in affinity chromatography?
A little background: as part of my bioinformatics degree I have to take Protein Chemistry course, but I miss chemistry basics (have CS background), so that's why I'm asking the following question.
We ...
- 139
3
votes
2
answers
810
views
What dictates cathode vs anode nomenclature use?
what was the justification for the use the cathode and anode terminology in IEF?
There is no redox taking place in IEF, just proteins interacting with an electric field
1
vote
0
answers
103
views
Protein purification pressure limits confusion, about the X-MPa limit for FPLC?
I have always been a bit confused about column pressure limits. I have to elaborate a bit on the problem to get to my question:
In protein purification we use the äkta purification machines (e.g. ...
- 918
1
vote
1
answer
4k
views
What does it mean to equilibrate a column in size exclusion chromatography and why is a buffer used?
I'm reading a procedure on size exclusion chromatography and it says that the first step is to equilibrate the column with buffer. What does this mean?
- 11
2
votes
2
answers
419
views
Why is the principle of separation in GFC opposite to electrophoresis despite using same material for the gel?
In GFC, larger proteins get eluted out first while in electrophoresis the larger proteins move slower. Why is this?
- 187
3
votes
1
answer
187
views
Gel filtration separation of cellulose, starch, oxytocin and palmitic acid
I am studying biochemistry and I have been asked by my teacher to find a procedure for separating cellulose, starch, the peptide oxytocin (9 a.a) and palmitic acid ($\ce{C16}$) using gel filtration ...
- 140
4
votes
1
answer
328
views
Reverse Phase Chromatography
Please help me figuring out what is going on with my RP-HPLC protocol.
I am purifying a small peptide out of E.coli Culture, heat stable enterotoxin, its amino acid sequence is:
N-T-F-Y-C-C-E-L-C-...
- 43