All Questions
Tagged with chromatography concentration
8
questions
1
vote
0
answers
71
views
"Is there any library in Python or software for analyzing raw chromatography data?"
I have taken the data directly from the FID sensor using a DAQ, so I have the chromatography data in this format:
(2 data colummns in a txt file) (18000 rows)
Time (s) Intensity (V)
0.00367___0.0008
...
- 27
0
votes
2
answers
2k
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Finding unknown concentration using HPLC standard curve
I calculated a standard curve equation from a given concentration and peak area, which gave me $y=1994.6x+218.4$. I made the graph with the $x$-axis μg/mL and $y$-axis peak area.
The question is, a ...
- 11
-1
votes
1
answer
107
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Chromatography- why tM is separated from other peaks? [closed]
tM in Chromatography is the time of exist mobile phase. But, the mobile phase exist together with all the compunds that tasted in the process. So, why in the graph of signal to time we can see a peak ...
- 11
1
vote
1
answer
1k
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Calculating the concentration of product in a solution based on the peak area of HPLC [closed]
Please help me resolving the following:
A sample solution, $\pu{0.24mg/mL}$ Compound A, is exposed to heat to form Compound B. From HPLC data report I have peak area for Compound A and Compound B. ...
1
vote
0
answers
48
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Column Dispersive Mass Balance analytical / numerical solution
Is there any analytical solution for the most common expression of column mass balance:
$\dfrac{\partial C}{\partial t} + \left(\dfrac{1+\varepsilon}{\varepsilon}\right)\dfrac{\partial q} {\partial t} ...
- 133
3
votes
2
answers
701
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Is it possible to calculate the concentration percentage using chromatography paper?
I'm trying to calculate the concentrations of specific compounds using paper chromatography. Would I be able to calculate a rough estimate by comparing the size of each concentrated spot? If not is ...
- 33
1
vote
2
answers
697
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Calculate concentration gradients in FPLC
Our FPLC works with two solutions:
A: 20 mM Tris.HCl, 0.2 M NaCl, pH 7.8
B: 20 mM Tris.HCl, 1.6 M NaCl, pH 7.8
I'm interested in sample volume from 102 mL to 117 mL. The FPLC will have a linear ...
- 662
3
votes
2
answers
2k
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Plotting a calibration curve for Gas-Liquid Chromatography
Question:
The given graph of abundance of different samples of butanol with respect to time is-
Textbook solution:
But why aren't the points like this:
$(20,5);(40,10);(60,15);(a,7)$
after which, ...
- 751