Questions tagged [fluorescence]
For questions relating to the radiation emitted by substances as a result of incident radiation.
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Spectroscopic methods for quantifying peptides/proteins with or without Tryptophan or Tyrosine content
I have several peptides (20-50 amino acids long) which I want to quantify the solubility/concentration in a solvent at certain temperature and pH.
These peptides may or may not contain Tryptophan or ...
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How can I save an EEM file on a Fluoromax4?
I'm trying to run excitation-emission matrices on some water samples, but I'm running into some software issues that the manuals aren't helpful for. In order to use R studio to extract my data, I need ...
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What is the amount of UV light required to make fluorophores glow and what is the minimum amount of electricity needed to achieve this?
So I have heard that fluorophores glow under UV light, so I was wondering what is the minimum amount of UV light needed to make the fluorophores glow and what would be the minimum amount of ...
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Microcopy Fluorescence filter set usefulness
I have a brief doubt and maybe someone around here can give as some help. We are doing a pilot study on microplastics using a fluorescence method (red nile). The dye is solvachromic, so it's emission ...
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Why does the emission of dansyl group diminish with time in this experiment?
I don't know how to make sense of this. I what is happening when an enzyme (carboxypeptidase, which contains tryptophan as its only chromophore and uses a Zn(II) ion in its active center) hydrolises a ...
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Fluorometer for measuring fluorescence in a sheet of water
Does anyone know of a fluorometer that I could use to measure fluorescence in a thin sheet of water. So it would be like holding a piece of writing or printer paper vertically and trying to get a ...
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Common chemcials that produce phosphorescence
Are there any common lab chemicals or household stuff that produce phosphorescence?
I am aware of ZnS, CaS, Fluorescein, Erythrosin B, Vanillin Benzaldehyde, Vitamin B2, Tryptophan etc.
I just need ...
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Choosing the right wavelength for calibration curve and for analysis of actual results
Introduction:
I am currently conducting a series of experiments in which I am studying the transport of polystyrene nanoparticles (PNPs) through sand and soil. This is done in sand/soil columns, into ...
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Does the laser side of laser fluorescence spectroscopy have to be so complicated?
I understand the working principle behind zapping some gas and looking at spectral emissions, and I can see why photodetection is so difficult. But I thought that pointing a laser into a vacuum ...
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Strange Binding Curve DNA:Protein Interaction
So I did some DNA:Protein Interaction studies using Fluorescence Polarisation Assays. I have fluorescently labelled DNA and add my Protein of interest. Down below you see an exemplary plot of the ...
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Dark smudge at the TLC solvent front
I have recently started using TLC in my graduate work to test for the cleavage of a cyclic oligo-adenylate in an enzymatic reaction. The sample I'm spotting on my plate is in an aqueous solution ...
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Why is there a mirror image effect in the absorbance and fluorescence spectra?
I've read some answers online but I still can't seem to understand the mirror image effect. Why is the highest energy absorption (v" = 0 to v' = 4) the lowest energy fluorescence (v' = 4 to v&...
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Which commonly available UV light source to use with fluorescein
I want to start experimenting with fluorescein. I have a 353 nm UV light source, but am thinking that it might be too short since it looks like peak excitation occurs at 494 nm. So am I correct in ...
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When an electron is excited to a singlet state, must it flip its spin state to enter the triplet state and flip once more to the ground state?
I understand the Jablonski diagram in that it has intersystem crossing from the singlet state to the triplet state, but how many spin switches are necessary to complete to phosphores? When an electron ...
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Is the activation energy always inversely proportional to the reaction rate?
When I obtained the activation energy using the Arrhenius equation for a 10-minute glowstick (rapid, rigorous reaction), it was almost 4 times that of a 12-hour glowstick (slow, mild reaction).
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