All Questions
Tagged with fluorescence spectroscopy
4
questions with no upvoted or accepted answers
3
votes
0
answers
99
views
How can I set up and process my excitation-emission matrices properly?
I'm running EEMS on some water samples on a Horiba Fluoromax 4 spectrofluorometer. Method below.
$\pu{240-580nm}$ emission, $\pu{5nm}$ interval, $\pu{1nm}$ width.
$\pu{240-480nm}$ excitation, $\pu{...
- 61
1
vote
0
answers
83
views
Spectroscopic methods for quantifying peptides/proteins with or without Tryptophan or Tyrosine content
I have several peptides (20-50 amino acids long) which I want to quantify the solubility/concentration in a solvent at certain temperature and pH.
These peptides may or may not contain Tryptophan or ...
- 189
1
vote
0
answers
39
views
Does the laser side of laser fluorescence spectroscopy have to be so complicated?
I understand the working principle behind zapping some gas and looking at spectral emissions, and I can see why photodetection is so difficult. But I thought that pointing a laser into a vacuum ...
- 111
0
votes
0
answers
73
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Does something like a fluorescent foil or a fluorescent film exist?
During my experiments I want the emitted laser beams to come back with a different wavelength than the original wavelength using a fluorescent foil or fluorescent film.
The laser I'm currently using ...
- 9