All Questions
Tagged with equilibrium biochemistry
18
questions
1
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0
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43
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Drug dosage determination using dynamical systems
I am a Mathematical Biology student, and recently I have read about various approaches, such as Ordinary Differential Equations (ODEs), used to determine the exact drug dosage and treatment sessions ...
- 11
0
votes
1
answer
84
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Isoelectric Point
How do we derive the formula of isoelectric point?
pI=(pKa1 + pKa2)/2
I read that isoelectric point is defined as the pH at which degree of protonation in amino group is same as degree of ...
0
votes
2
answers
77
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Why does a small decrease in [ATP] cause a large increase in [AMP]
I was wondering how the reaction catalysed by adenylate kinase and its equilibrium of approximately 1 leads to [AMP] changing greatly with a small change in [ATP]. The below information was provided ...
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-3
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2
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1k
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How to determine the charge of amino acid at certain pH? [duplicate]
Problem
What would charge would you expect on alanine when placed in a solution with a pH of 1.00?
Answer
Question
Let's say I am given a certain pH of 2.00 rather than 1.00 for the "acidic ...
- 11
3
votes
1
answer
191
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Constants in Enzyme Kinetics under the Rapid Equilibrium Assumptions
I am new to enzyme kinetics, and I am trying to write an rate equation for a rapid equilibrium random terreactant system. I have been consulting the books Enzyme Kinetics and Mechanism by Cook and ...
1
vote
1
answer
234
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What is an alternate way of preparing a sodium hydrogen phosphate/sodium dihydrogen phosphate buffer through titrating an acid with a strong base?
So my friend and I are stumped. This is in the context of achieving the sodium hydrogen phosphate/sodium
dihydrogen phosphate buffer by titrating the acid with a strong base.
My friend says the answer ...
- 19
1
vote
1
answer
180
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How to derive the steady-state solution for simple two-step reaction with differential equations? [closed]
Consider this simple two step reaction, a variant of a Michaelis-Menten type of problem, where $\ce{A}$ and $\ce{B}$ reversibly bind to make $\ce{AB}$, and $\ce{AB}$ and $\ce{C}$ reversibly bind to ...
- 113
0
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118
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How can the ocean act as a buffer?
I was reading up on the buffering effect of the ocean and I was quite confused about how the buffer is able to do anything. The buffer I will refer to is below:
$$\ce{CO3^{-2} +H2O <=> HCO3 + OH^...
- 1,584
2
votes
1
answer
92
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Relative fraction of population of different enzyme conformations, knowing the rate constants
Say we have an enzyme that has multiple structural conformations, say A, B, C and D. Additionally, we say that the enzyme must go through all conformations, to get to one end to the other, i.e.
$$\...
- 123
3
votes
0
answers
89
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Formating chemical equations for proteins binding in multiple configurations
I am working on problems involving protein-protein binding, particularly ones in which two proteins may bind in two or more configurations, and where some of the resultant structures may also bind ...
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16
votes
2
answers
8k
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Is the Gibbs standard free energy always constant?
I am a biochemistry student and we are learning about thermodynamics. Is the Gibbs standard free energy for a reaction always constant? The equation below suggests that it changes with temperature:
$$...
- 455
1
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0
answers
309
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Hemoglobin equilibrium - effect of increased carbon dioxide and lactic acid
The binding of oxygen by haemoglobin giving oxy-haemoglobin is partially regulated by the concentration of $\ce{H3O+}$ and dissolved in $\ce{CO2}$ in blood
$$\ce{HbO2 + H3O+ +CO2<=>H+.Hb.CO2 + ...
user14857
2
votes
1
answer
2k
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Why don't ions have one equilibrium potential? (Nernst equation)
I know that equilibrium potentials are dependent on the ratio of ion concentrations inside and outside of the cell and temperature. I also know that the equilibrium potential is reached when there is ...
- 56
12
votes
4
answers
1k
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How can a protein folding transition state have zero lifetime?
I'm doing a module on my Biochemistry course looking at protein folding, and in a discussion of [folding] transition states I was a little confused at the thought of a zero-lifetime transition state - ...
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3
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1
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4k
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Units for dissociation constant and relationship to Gibbs Free Energy
How does one "convert" between an equilibrium constant calculated from the Gibbs free energy for a dissociation reaction and experimentally determined dissociation constants?
$$
\Delta G^\circ=-RT\...
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