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GREEN SYNTHESIS OF MAGNETIC NANOPARTICLES
AND THEIR BIOLOGICALAPPLICATIONS
PRESENTED BY: AHMEDSAEED
SUPERVISED BY: PROFESSORDR.AKRAMRAZA
1
Types of Magnetic Nanoparticles
TEM-images-of-cobalt-ferrite--NPs
FE-SEM images: (a, b) for CoFe2O4 samples
and (c, d) for NiFe2O4 samples
 Iron Oxide Nanoparticles
 Magnetite (Fe3O4)
 Maghemite (γ-Fe2O3)
 Metallic Nanoparticles
 Iron (Fe)
 Cobalt (Co)
 Alloy Nanoparticles
 Iron-Platinum (FePt)
 Cobalt-Platinum (CoPt)
 Ferrite Nanoparticles
 Nickel Ferrite (NiFe2O4)
 Cobalt Ferrite (CoFe2O4)
2
Properties of Magnetic Nanoparticles
 Super-Paramagnetism:
 No remnant magnetization.
 Surface Functionalization:
 Modifiable for specific uses.
 Biocompatibility:
 Safe for biological systems.
 Chemical Stability:
 Resistant to oxidation and degradation.
 Thermal Stability:
 Maintains properties under varying temperatures.
3
Introduction to Green Synthesis
 Definition
 Biological Organisms:
 Use of plants, microbes, enzymes.
 Origins
 Eco-friendly chemical production reducing harmful substances.
 Developed for sustainable and safe chemical processes.
 Core Principles
 Uses renewable resources.
 Safer solvents and conditions.
 Reduces waste and energy use.
 Significance
 Promotes sustainability and safety.
 Supports global pollution reduction efforts.
 Applications
 Pharmaceuticals, nanotechnology, agriculture.
4

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This document summarizes a student project on the green synthesis of nanoparticles. It discusses various methods for synthesizing nanoparticles, emphasizing that green synthesis is more eco-friendly than physical or chemical methods as it does not require high temperatures, pressures, or toxic chemicals. The document then describes how plant extracts can be used to synthesize nanoparticles and the characterization techniques used to analyze the particles produced, including UV-vis spectroscopy, DLS, SEM, TEM and FTIR. It concludes by noting some applications of green-synthesized nanoparticles in fields such as medicine, environment and engineering.

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The next years will prove the importance of greensynthesis methods for MNPs and MONPs production because they are not only easy to execute, fast, and cheap but also less toxic and environmentally ecofriendly. Nanoparticle synthesis using microorganisms and plants by green synthesis technology is biologically safe, cost-effective, and environment-friendly. Plants and microorganisms have established the power to devour and accumulate inorganic metal ions from their neighboring niche. The biological entities are known to synthesize nanoparticles bothextra and intracellularly. The capability of a living system to utilize its intrinsic organic chemistry processes in remodeling inorganic metal ions into nanoparticles has opened up an undiscovered area of biochemical analysis. Metal nanoparticles (MNPs) and metal oxidenanoparticles (MONPs) are used in numerous fields. The new nano-based entities are being strongly generated and incorporated into everyday personal care products, cosmetics, medicines, drug delivery, and clothing toimpact industrial and manufacturing sectors, which means that nanomaterials commercialization and nanoassisted device will continuously grow. They can be prepared by many methods such as green synthesis and the conventional chemical synthesis methods. The green synthesis of nanoparticles (NPs) using living cells is a promising and novelty tool in bionanotechnology. Chemical and physical methods are used to synthesize NPs; however, biological methods are preferred due to its eco-friendly, clean, safe, cost effective, easy, and effective sources for high productivity and purity. Greensynthesis includes infinite accession to produce MNPs and MONPs with demanding properties. The structure–function relationships between nanomaterials and key information for life cycle evaluation lead to the production of high execution nanoscale materials that are gentle and environmentally friendly. Majority of plants have features as sustainable and renewable suppliers compared with microbes and enzymes, as they have the ability to pick up almost 75% of the light energy and transform it into chemical energy, contain chemicals like antioxidants and sugars, and play fundamental roles in the manufacture of nanoparticles. Plants considered the main factory for the green synthesis of MNPs and MONPs, and until now, different plant species have been used to study this, but the determined conditions should be taken into consideration to execute this preparation.

eco-friendly green biosynthes
Why Green Synthesis?
 Renewable Resources:
 Use of natural, sustainable materials.
 Energy Efficiency:
 Often requires lower energy input.
 Biocompatibility:
 Produces non-toxic nanoparticles.
 Public Health:
 Safer for researchers and consumers.
 Regulatory Compliance:
 Easier to meet environmental regulations.
 Sustainability:
 Utilizes renewable resources.
 Cost-effectiveness:
 Often more economical.
5
Methods of Green Synthesis
 Plant Extracts
 Natural reducers
 Example: Leaf extracts
 Microbial Synthesis
 Bacteria, fungi, algae
 Example: Fusarium oxysporum
 Biopolymers
 Natural templates
 Example: Chitosan
 Enzymatic Synthesis
 Biocatalysts
 Example: Enzyme-mediated
 Aqueous Phase Synthesis
 Water-based reactions
 Example: Hydrothermal
6
Plant Extracts in Synthesis
 Neem (Azadirachta indica)
 Widely used for synthesizing silver and gold nanoparticles.
 Contains bioactive compounds like azadirachtin.
 Tea (Camellia sinensis)
 Rich in polyphenols and antioxidants.
 Effective in producing stable gold and silver nanoparticles.
 Eucalyptus (Eucalyptus globulus)
 High concentration of essential oils.
 Used in synthesizing a variety of metal nanoparticles.
 Banana (Musa paradisiaca)
 Contains reducing sugars and phenolic compounds.
 Utilized for the synthesis of silver and gold nanoparticles.
 Mango (Mangifera indica)
 Rich in flavonoids and phenolic compounds.
 Effective in producing gold nanoparticles.
 Hibiscus (Hibiscus rosa-sinensis)
 Contains anthocyanins and flavonoids.
 Used for synthesizing silver nanoparticles.
7
Microbial Synthesis
 Bacteria
 Examples: Pseudomonas aeruginosa, Bacillus subtilis.
 Role: Act as reducing agents to convert metal ions to nanoparticles.
 Fungi
 Examples: Aspergillus niger, Fusarium oxysporum.
 Role: Secrete enzymes and proteins that facilitate nanoparticle synthesis.
 Algae
 Examples: Chlorella vulgaris, Spirulina platensis.
 Role: Utilize photosynthesis to reduce metal ions to nanoparticles.
 Yeasts
 Examples: Saccharomyces cerevisiae, Candida utilis.
 Role: Produce bioactive molecules that aid in nanoparticle formation.
 Actinomycetes
 Examples: Streptomyces spp., Nocardia spp.
 Role: Produce extracellular enzymes for nanoparticle synthesis.
 Mechanism
 Process: Metal ions are reduced to nanoparticles by microbial metabolites.
 Stabilization: Microbial proteins and enzymes cap and stabilize nanoparticles.
8

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Enzymatic Processes
 Introduction to Enzymatic Synthesis
 Utilizes enzymes as catalysts for eco-friendly production of
metal nanoparticles, offering precise control over size and
shape.
 Types of Enzymes Used
 Enzymes like oxidoreductases (e.g., peroxidases) and lyases (e.g., phytases)
are employed for their ability to reduce metal ions and stabilize resulting
nanoparticles.
 Mechanism of Enzymatic Reduction
 Enzymes facilitate reduction reactions, converting metal ions into
nanoparticles by controlling nucleation and growth processes.
��� Advantages of Enzymatic Synthesis
 Benefits include biocompatibility, operation under mild conditions (neutral
pH, room temperature), and the avoidance of harsh chemicals typically used
in conventional methods.
9
Case Study: Tea Extract Synthesis
 Process:
 Iron oxide nanoparticles synthesized using tea extract.
 Results:
 High yield, good magnetic properties.
 Advantages:
 Simple, cost-effective, eco-friendly.
 Applications:
 Drug delivery, MRI contrast agents.
10
Advantages of Microbial Synthesis
 Eco-friendly:
 Uses natural microorganisms.
 Scalability:
 Potential for large-scale production.
 Specificity:
 Genetically engineered for specific synthesis.
 Biocompatibility:
 Produces non-toxic nanoparticles.
 Efficiency:
 High yield under optimized conditions.
 Research Focus:
 Enhancing microbial synthesis pathways
11
Case Study: Bacterial Synthesis (magnetotactic bateria)
 Metal Ion Uptake:
 Bacteria absorb metal ions like Fe(II) and Fe(III) from their
surroundings.
 Intracellular Transformation:
 Enzymatic reduction converts absorbed ions to ferrous (Fe2+)
and then ferric (Fe3+) forms inside the cell.
 Nucleation of Nanoparticles:
 Ferric ions nucleate within magnetosomes, specialized cell
compartments.
 Crystal Growth and Maturation:
 Nanoparticles grow and crystallize into magnetite or greigite
under protein control.
 Biomineralization Control:
 Proteins regulate nanoparticle size, shape, and magnetic
properties.
12

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Biological Applications of MNPs
 Drug Delivery:
 Targeted delivery using magnetic fields.
 Hyperthermia Treatment:
 MNPs generate heat to kill cancer cells.
 Magnetic Resonance Imaging (MRI):
 Contrast agents for improved imaging.
 Biosensors:
 Detection of biomolecules for diagnostics.
 Environmental Applications:
 Removal of pollutants from water.
 Tissue Engineering:
 Scaffold design and regenerative medicine.
13
Drug Delivery Systems
 Mechanism:
 MNPs loaded with drugs can be directed to specific sites using
magnetic fields.
 Benefits:
 Targeted therapy, reduced side effects.
 Controlled Release:
 Precise delivery of therapeutic agents.
 Biocompatibility:
 Safe for use in vivo.
 Examples:
 Chemotherapeutics, antibiotics.
14
Biosensors
 Mechanism:
 MNPs functionalized with specific biomolecules for detecting pathogens or
biomarkers.
 Applications:
 Diagnostics, environmental monitoring, food safety.
 Advantages:
 High sensitivity, rapid detection.
15
Hyperthermia Treatment
 Mechanism: MNPs
 generate heat when exposed to an alternating magnetic field.
 Application:
 Localized heating to kill cancer cells.
 Advantages:
 Minimally invasive, precise targeting.
 Challenges:
 Controlling temperature and avoiding damage to healthy tissue.
 Research:
 Optimizing MNPs for maximum heat generation.
 Clinical Trials:
 Exploring effectiveness in various cancer types.
16

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MRI Contrast Agents
 Mechanism:
 MNPs improve contrast in MRI scans.
 Beznefits:
 nhanced imaging of tissues and organs.
 Applications:
 Diagnosis of diseases, monitoring treatment progress.
 Advantages:
 High contrast, biocompatibility.
 Challenges:
 Ensuring long-term stability and safety.
 Research:
 Developing MNPs with specific targeting abilities.
17
Environmental Applications
 Mechanism:
 MNPs used to remove pollutants like heavy metals from water.
 Benefits:
 Effective, reusable, eco-friendly.
 Applications:
 Water purification, soil remediation.
 Advantages:
 High efficiency, low cost.
 Future Directions:
 Developing MNPs for specific pollutants.
18
Challenges in Green Synthesis of
Magnetic Nanoparticles
 Control over Particle Size and Morphology
 struggle to achieve precise control over the size and morphology of magnetic
nanoparticles.
 This variability can affect their magnetic properties and application suitability.
 Scalability
 At industrial scale efficiency and cost effectiveness remain a challenge
 Stability and Shelf Life:
 exhibit lower stability or shorter shelf life compared to their conventionally synthesized
counterparts
 Surface Functionalization and Biocompatibility:
 adequate surface functionalization without compromising their green nature and
biocompatibility is a significant challenge.
19
Future Directions
 Enhancing Efficiency and Scalability
 Develop improved, efficient and scalable green synthesis methods
 Exploring Novel Green Resources
 Investigate new bioresources and waste mate
 Functionalization and Surface Engineering:
 Develop methods for precise surface functionalization
 Integration with AI and Computational Approaches
 Utilize artificial intelligence (AI) and computational modeling to accelerate the design
and optimization of green synthesis methods.
 Collaborative Research and Knowledge Sharing:
 Foster interdisciplinary collaborations between chemists, biologists, engineers, and
environmental scientists to advance knowledge sharing and innovation in green
synthesis technologies.
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To My Guiding Star
This presentation is dedicated to
The Legend Behind My Success,
Sir Dr Prof. AKRAM RAZA.
whose unwavering guidance and support
have been the foundation of my success.
Your inspiration and encouragement mean
the world to me.
The Architect of My Accomplishments,
Pictured Here!
21
Gratitude Overload!
Special kudos to you all
your patience deserves a medal!
22

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Green Synthesis of Magnetic Nanoparticles and Their Biological application.pptx

  • 1. GREEN SYNTHESIS OF MAGNETIC NANOPARTICLES AND THEIR BIOLOGICALAPPLICATIONS PRESENTED BY: AHMEDSAEED SUPERVISED BY: PROFESSORDR.AKRAMRAZA 1
  • 2. Types of Magnetic Nanoparticles TEM-images-of-cobalt-ferrite--NPs FE-SEM images: (a, b) for CoFe2O4 samples and (c, d) for NiFe2O4 samples  Iron Oxide Nanoparticles  Magnetite (Fe3O4)  Maghemite (γ-Fe2O3)  Metallic Nanoparticles  Iron (Fe)  Cobalt (Co)  Alloy Nanoparticles  Iron-Platinum (FePt)  Cobalt-Platinum (CoPt)  Ferrite Nanoparticles  Nickel Ferrite (NiFe2O4)  Cobalt Ferrite (CoFe2O4) 2
  • 3. Properties of Magnetic Nanoparticles  Super-Paramagnetism:  No remnant magnetization.  Surface Functionalization:  Modifiable for specific uses.  Biocompatibility:  Safe for biological systems.  Chemical Stability:  Resistant to oxidation and degradation.  Thermal Stability:  Maintains properties under varying temperatures. 3
  • 4. Introduction to Green Synthesis  Definition  Biological Organisms:  Use of plants, microbes, enzymes.  Origins  Eco-friendly chemical production reducing harmful substances.  Developed for sustainable and safe chemical processes.  Core Principles  Uses renewable resources.  Safer solvents and conditions.  Reduces waste and energy use.  Significance  Promotes sustainability and safety.  Supports global pollution reduction efforts.  Applications  Pharmaceuticals, nanotechnology, agriculture. 4
  • 5. Why Green Synthesis?  Renewable Resources:  Use of natural, sustainable materials.  Energy Efficiency:  Often requires lower energy input.  Biocompatibility:  Produces non-toxic nanoparticles.  Public Health:  Safer for researchers and consumers.  Regulatory Compliance:  Easier to meet environmental regulations.  Sustainability:  Utilizes renewable resources.  Cost-effectiveness:  Often more economical. 5
  • 6. Methods of Green Synthesis  Plant Extracts  Natural reducers  Example: Leaf extracts  Microbial Synthesis  Bacteria, fungi, algae  Example: Fusarium oxysporum  Biopolymers  Natural templates  Example: Chitosan  Enzymatic Synthesis  Biocatalysts  Example: Enzyme-mediated  Aqueous Phase Synthesis  Water-based reactions  Example: Hydrothermal 6
  • 7. Plant Extracts in Synthesis  Neem (Azadirachta indica)  Widely used for synthesizing silver and gold nanoparticles.  Contains bioactive compounds like azadirachtin.  Tea (Camellia sinensis)  Rich in polyphenols and antioxidants.  Effective in producing stable gold and silver nanoparticles.  Eucalyptus (Eucalyptus globulus)  High concentration of essential oils.  Used in synthesizing a variety of metal nanoparticles.  Banana (Musa paradisiaca)  Contains reducing sugars and phenolic compounds.  Utilized for the synthesis of silver and gold nanoparticles.  Mango (Mangifera indica)  Rich in flavonoids and phenolic compounds.  Effective in producing gold nanoparticles.  Hibiscus (Hibiscus rosa-sinensis)  Contains anthocyanins and flavonoids.  Used for synthesizing silver nanoparticles. 7
  • 8. Microbial Synthesis  Bacteria  Examples: Pseudomonas aeruginosa, Bacillus subtilis.  Role: Act as reducing agents to convert metal ions to nanoparticles.  Fungi  Examples: Aspergillus niger, Fusarium oxysporum.  Role: Secrete enzymes and proteins that facilitate nanoparticle synthesis.  Algae  Examples: Chlorella vulgaris, Spirulina platensis.  Role: Utilize photosynthesis to reduce metal ions to nanoparticles.  Yeasts  Examples: Saccharomyces cerevisiae, Candida utilis.  Role: Produce bioactive molecules that aid in nanoparticle formation.  Actinomycetes  Examples: Streptomyces spp., Nocardia spp.  Role: Produce extracellular enzymes for nanoparticle synthesis.  Mechanism  Process: Metal ions are reduced to nanoparticles by microbial metabolites.  Stabilization: Microbial proteins and enzymes cap and stabilize nanoparticles. 8
  • 9. Enzymatic Processes  Introduction to Enzymatic Synthesis  Utilizes enzymes as catalysts for eco-friendly production of metal nanoparticles, offering precise control over size and shape.  Types of Enzymes Used  Enzymes like oxidoreductases (e.g., peroxidases) and lyases (e.g., phytases) are employed for their ability to reduce metal ions and stabilize resulting nanoparticles.  Mechanism of Enzymatic Reduction  Enzymes facilitate reduction reactions, converting metal ions into nanoparticles by controlling nucleation and growth processes.  Advantages of Enzymatic Synthesis  Benefits include biocompatibility, operation under mild conditions (neutral pH, room temperature), and the avoidance of harsh chemicals typically used in conventional methods. 9
  • 10. Case Study: Tea Extract Synthesis  Process:  Iron oxide nanoparticles synthesized using tea extract.  Results:  High yield, good magnetic properties.  Advantages:  Simple, cost-effective, eco-friendly.  Applications:  Drug delivery, MRI contrast agents. 10
  • 11. Advantages of Microbial Synthesis  Eco-friendly:  Uses natural microorganisms.  Scalability:  Potential for large-scale production.  Specificity:  Genetically engineered for specific synthesis.  Biocompatibility:  Produces non-toxic nanoparticles.  Efficiency:  High yield under optimized conditions.  Research Focus:  Enhancing microbial synthesis pathways 11
  • 12. Case Study: Bacterial Synthesis (magnetotactic bateria)  Metal Ion Uptake:  Bacteria absorb metal ions like Fe(II) and Fe(III) from their surroundings.  Intracellular Transformation:  Enzymatic reduction converts absorbed ions to ferrous (Fe2+) and then ferric (Fe3+) forms inside the cell.  Nucleation of Nanoparticles:  Ferric ions nucleate within magnetosomes, specialized cell compartments.  Crystal Growth and Maturation:  Nanoparticles grow and crystallize into magnetite or greigite under protein control.  Biomineralization Control:  Proteins regulate nanoparticle size, shape, and magnetic properties. 12
  • 13. Biological Applications of MNPs  Drug Delivery:  Targeted delivery using magnetic fields.  Hyperthermia Treatment:  MNPs generate heat to kill cancer cells.  Magnetic Resonance Imaging (MRI):  Contrast agents for improved imaging.  Biosensors:  Detection of biomolecules for diagnostics.  Environmental Applications:  Removal of pollutants from water.  Tissue Engineering:  Scaffold design and regenerative medicine. 13
  • 14. Drug Delivery Systems  Mechanism:  MNPs loaded with drugs can be directed to specific sites using magnetic fields.  Benefits:  Targeted therapy, reduced side effects.  Controlled Release:  Precise delivery of therapeutic agents.  Biocompatibility:  Safe for use in vivo.  Examples:  Chemotherapeutics, antibiotics. 14
  • 15. Biosensors  Mechanism:  MNPs functionalized with specific biomolecules for detecting pathogens or biomarkers.  Applications:  Diagnostics, environmental monitoring, food safety.  Advantages:  High sensitivity, rapid detection. 15
  • 16. Hyperthermia Treatment  Mechanism: MNPs  generate heat when exposed to an alternating magnetic field.  Application:  Localized heating to kill cancer cells.  Advantages:  Minimally invasive, precise targeting.  Challenges:  Controlling temperature and avoiding damage to healthy tissue.  Research:  Optimizing MNPs for maximum heat generation.  Clinical Trials:  Exploring effectiveness in various cancer types. 16
  • 17. MRI Contrast Agents  Mechanism:  MNPs improve contrast in MRI scans.  Beznefits:  nhanced imaging of tissues and organs.  Applications:  Diagnosis of diseases, monitoring treatment progress.  Advantages:  High contrast, biocompatibility.  Challenges:  Ensuring long-term stability and safety.  Research:  Developing MNPs with specific targeting abilities. 17
  • 18. Environmental Applications  Mechanism:  MNPs used to remove pollutants like heavy metals from water.  Benefits:  Effective, reusable, eco-friendly.  Applications:  Water purification, soil remediation.  Advantages:  High efficiency, low cost.  Future Directions:  Developing MNPs for specific pollutants. 18
  • 19. Challenges in Green Synthesis of Magnetic Nanoparticles  Control over Particle Size and Morphology  struggle to achieve precise control over the size and morphology of magnetic nanoparticles.  This variability can affect their magnetic properties and application suitability.  Scalability  At industrial scale efficiency and cost effectiveness remain a challenge  Stability and Shelf Life:  exhibit lower stability or shorter shelf life compared to their conventionally synthesized counterparts  Surface Functionalization and Biocompatibility:  adequate surface functionalization without compromising their green nature and biocompatibility is a significant challenge. 19
  • 20. Future Directions  Enhancing Efficiency and Scalability  Develop improved, efficient and scalable green synthesis methods  Exploring Novel Green Resources  Investigate new bioresources and waste mate  Functionalization and Surface Engineering:  Develop methods for precise surface functionalization  Integration with AI and Computational Approaches  Utilize artificial intelligence (AI) and computational modeling to accelerate the design and optimization of green synthesis methods.  Collaborative Research and Knowledge Sharing:  Foster interdisciplinary collaborations between chemists, biologists, engineers, and environmental scientists to advance knowledge sharing and innovation in green synthesis technologies. 20
  • 21. To My Guiding Star This presentation is dedicated to The Legend Behind My Success, Sir Dr Prof. AKRAM RAZA. whose unwavering guidance and support have been the foundation of my success. Your inspiration and encouragement mean the world to me. The Architect of My Accomplishments, Pictured Here! 21
  • 22. Gratitude Overload! Special kudos to you all your patience deserves a medal! 22