First off, you will need a minimum of three 2-digit numbers, because none of 1, 4, 6, 8, or 9 is prime, so they will need to be combined with another digit in order to make a prime number.
It quickly becomes evident that if there are three 2-digit numbers, it is impossible to combine them in such a way that they "cancel out", and allow an equation that works with the three much-smaller remaining digits.
Thus it is necessary to create four 2-digit numbers. These numbers will all necessarily be odd, since the only even prime is 2, which is a single digit.
The 2-digit primes can't end with 5, so the only ending digits available are 1, 3, 7, 9.
Any adding or subtracting of four odd numbers will always leave you with an even number, so the single-digit number must be 2.
Then it is just a matter of playing around with a few combinations of two-digit numbers that start with {4, 5, 6, 8} and end with {1, 3, 7, 9} to end up with:
$$67 + 59 - 83 - 41 = 2$$