Suppose there is a solution of enzyme, its substrate, a competitive inhibitor, and a suicide inhibitor. The reaction rate constant for the reaction of the suicide inhibitor with the enzyme is known. The Ki for the competitive inhibitor is also known. The occupation of the enzyme's active site by the competitive inhibitor prevents deactivation of the enzyme by the suicide inhibitor. The initial concentrations of the inhibitors are also known. How would the presence of the competitive inhibitor affect the observed reaction rate constant for the inactivation of the enzyme by the suicide inhibitor in the following cases:
- When the enzyme has been inactivated by the suicide inhibitor, the competitive inhibitor cannot enter the enzyme's active site.
- When the enzyme has been inactivated by the suicide inhibitor, a molecule of the competitive inhibitor can still enter the enzyme's active.
- A molecule of the competitive inhibitor can still enter an inactivated enzyme's active site, but at a lower probability compared to that of the active enzyme.
Is there an equation to determine the observed inhibition reaction rate constant based on the actual rate constant, the Ki of the competitive inhibitor, and the concentrations of the enzyme and the inhibitors.