All Questions
6
questions
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Strange Binding Curve DNA:Protein Interaction
So I did some DNA:Protein Interaction studies using Fluorescence Polarisation Assays. I have fluorescently labelled DNA and add my Protein of interest. Down below you see an exemplary plot of the ...
- 488
3
votes
0
answers
38
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Assessing stability of protein used for interaction experiments after changing buffer from tris to HEPES
I have a protein with a theoretical $\mathrm{pI}~9.0$ currently stored in $\pu{25 mM}$ tris and $\pu{250 mM}$ $\ce{NaCl}$ $(\mathrm{pH}~8)$ at $\pu{−80 °C}.$ I need to do experiments in HEPES at $\...
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1
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0
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36
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What do natural hybrids of peptides and nucleic acids look like?
Until recently I thought that there was a clean distinction between polypeptides and oligonucleotides, but apparently there are recent studies of therapeutic compounds that could be described as "...
- 11
3
votes
1
answer
1k
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Which is more stable chemically: DNA or peptide
Assuming enzyme-free environment, and the DNA and peptide are lyophillized, and then stored in vials under the same conditions (temperature and humidity).
On average, which would be more chemically ...
- 419
3
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1
answer
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Gel electrophoresis separates proteins on the basis of what property?
In SDS-PAGE for separation of proteins, SDS is an ionic detergent that coats the unfolded protein to mask its native charge and give at a uniform charge to length ratio. Since the length of the ...
- 422
3
votes
1
answer
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Denaturing of Proteins and Nucleic Acids - Effect of Temperature (Heating vs Cooling)
Heating a protein/nucleic acid will disrupt inter-molecular and intra-molecular forces in the tertiary structure. It will also interfere with the shape of the active site if the protein.
Does this ...
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