All Questions
3
questions
1
vote
0
answers
460
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Choosing the right wavelength for calibration curve and for analysis of actual results
Introduction:
I am currently conducting a series of experiments in which I am studying the transport of polystyrene nanoparticles (PNPs) through sand and soil. This is done in sand/soil columns, into ...
- 1,410
2
votes
1
answer
2k
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Is Beer-Lambert law also valid for fluorescence and luminescence?
When I measure optical density in a microplate reader the absorbance is proportional to the concentration and the height of the liquid. Is Beer-Lambert law also valid for fluorescence and luminescence?...
- 123
3
votes
1
answer
597
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Why do fluorescence intensities decline with replicate number?
I am performing a determination of riboflavin with fluorescence spectrophotometry. I've dissolved my riboflavin in a dilute GAA solution, created a calibration curve and measured my unknown's ...
- 2,354