All Questions
Tagged with experimental-chemistry biochemistry
32
questions
4
votes
1
answer
121
views
What role does CaCl2 play in a gelation medium?
To produce sodium alginate beads coated with chitosan, following procedure was undertaken:
Sodium alginate was dissolved in distilled water at a concentration of 4% (...) The gelation medium was ...
- 43
11
votes
2
answers
3k
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Is nitroglycerin synthesis within human digestive system from eating soap possible?
Could you make nitroglycerin by eating large quantities of pure glycerine soap? A quick google search told me flatus are 59% nitrogen, but I couldn't find anyone else with my exact question (which ...
- 135
2
votes
0
answers
37
views
Glycation of peptides (Maillard reaction) by reductive amination
I would like to link glucose to the primary amines in a peptide containing lysine. Can I just mix glucose and the peptide and add some cyanoborohydride to perform a reductive amination? As long as my ...
- 137
2
votes
0
answers
1k
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Molarity 1% ammonia solution?
I have a stock solution of 25% ammonia, with a density of 910 g/L. I used this to make a 1% ammonia solution by mixing 20 mL 25% ammonia into 480 mL distilled water with 5.84 g NaCl (0.2 M), density ~...
- 31
2
votes
0
answers
69
views
Is there any way to measure or otherwise quantify the extent of caramelization experimentally?
I've been trying to design an experiment where I want to compare the efficacy of caramelization of a monosaccharide under different pH conditions (the sugar would be placed in buffers of varying pH). ...
1
vote
0
answers
33
views
Is LCP working well in real experiments? or is it more of an oversimplified principle?
I have a question for people who have applied Le Chatelier's principle (LCP) to their experiment or have knowledge about people who have done that to increase the yield.
Essentially, I am curious
&...
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-1
votes
1
answer
106
views
Will concentration of sodium hydroxide affect the result of chicken feather hydrolyzate if i don't standardized it?
The title of the paper:
Isolation and characterization of keratin from chicken feathers https://doi.org/10.1063/5.0002792
Here is the method:
Keratin was isolated by dissolving 5g of chicken feather ...
- 79
-4
votes
1
answer
103
views
How Bread is made with yeast, sugar and luke warm milk? [closed]
Materials and Apparatus:
wheat flour
sugar
dry yeast
glass bowl
covering plate
milk
Procedure:
Lukewarm milk is taken in the glass bowl and sugar is added to it. Then, yeast is added to the same.
...
- 101
0
votes
1
answer
53
views
Using samples in titrations
I've never done an experiment before. So, the methodology provided in this pdf is quite confusing.
Link to the pdf: https://www.canterbury.ac.nz/media/documents/science-outreach/vitaminc_iodine.pdf
So,...
- 65
3
votes
0
answers
223
views
Why neglecting Bradford Solution Volume in the assay?
I would like to know why do we not need to take account of Bradford agent solution in dilution calculation?
For example, $\pu{2.5 \mu g mL-1}$ BSA will calculate from $\pu{0.4 \mu L}$ of $\pu{1 mg mL-...
- 131
5
votes
1
answer
44
views
Carbon 11 radiolabeled inhaled Beta-2 agonist or corticosteroids, is it possible?
Background
Positron emission tomography (PET) is a highly sensitive imaging modality that can provide in vivo quantitative information of biological processes at a biochemical level. PET relies upon ...
- 51
0
votes
1
answer
46
views
Multiday lab courses in classic chemistry [closed]
This time of the year I usually get some extra income I can use on interesting courses. Usually rescue or sport courses. However, I also have a master degree in chemistry, and would like to spend some ...
1
vote
0
answers
54
views
To get clear plasma out of a minute particle (such as fibrin), is it necessary to vortex sample before centrifugation? [closed]
I'm going to analyse plasma samples that contain a lot of fibrin. It is necessary to centrifuge the samples to be able to pipette the plasma. After the samples are centrifuged I can't vortex them ...
- 11
-3
votes
1
answer
28
views
Can there be a compound which stops the pesticide from entering into the fruit or vegetable? [closed]
Can there be a compound which stops the pesticide from entering into the fruit or vegetable? For example, a solution which is sprayed before pesticides and somehow blocks pesticides from entering into ...
- 1
1
vote
0
answers
36
views
How to convert pepsin into powder form?
I have pepsin powder which is now hydrated due to humidity. I want to convert it back to the powder form. How to do it?
I don't have access to a freeze-dryer .
I prefer any cheap procedure other than ...
- 49