I need to stain some double-stranded DNA with an intercalating fluorescent dye. For imaging, I am using a microscope setup equiped with a 532 nm green laser.
What is the staining agent of choice to use with a 532 nm excitation source?
Sytox Orange may work but it is a bit pricey.
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1$\begingroup$ Did you try propidium iodide? It is less fluorescent than Sytox Orange, but cheap. $\endgroup$– Mathew MahindaratneCommented Mar 25, 2022 at 16:41
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$\begingroup$ @MathewMahindaratne It is certainly cheap enough and absorbs at the right wavelength, I will try that $\endgroup$– BrenllaCommented Mar 25, 2022 at 16:51
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1$\begingroup$ Check this as well: fishersci.com/shop/products/live-or-dye-594-614-50as/501964599 $\endgroup$– Mathew MahindaratneCommented Mar 25, 2022 at 16:54
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