I have heard from women (in mathematics departments, in the US) that they prefer not to be addressed as Ms or Mrs.
The issue is that some students address their male professors as "Dr." or "Professor" but their female professors as "Ms." or "Mrs." Perhaps you don't do this, but I would still recommend "Dr." or "Professor" unless your professor encourages otherwise.
Indeed, I know of one female professor who tells students that she is happy to be called "Dr. X" or "Professor X", and happier still to be addressed by her first name, but requests that her students avoid "Ms" or "Mrs".
It should also be added that this in the US, and cultural practices may be different elsewhere.
I don't think it's necessary to send a follow-up e-mail, but since you are worried about it, I think it would be perfectly polite to write something very brief like "p.s. How do you prefer to be addressed? Is Dr. X better?"