All Questions
30
questions
0
votes
3
answers
153
views
Imaging and illumination plane in microscope
I've been delving into the intricacies of achieving Kohler illumination in microscopy, and the term 'illumination and image-forming rays' consistently surfaces. Most resources emphasize the ...
1
vote
2
answers
138
views
Trouble simulating/ray tracing a simple microscope
I'm trying to run a (seemingly) simple simulation of a basic microscope using the free software WinLens 3D Basic, but the ray tracing looks wrong:
The microscope consists of a small plano-convex lens ...
- 181
0
votes
1
answer
135
views
How can I magnify an infinity corrected image onto a camera surface?
I know that the typical setup with a camera will place the CMOS sensor at I(3) in the image below, and that this magnification is dictated by the tube lens focal ...
- 101
2
votes
3
answers
78
views
Operation of a microscope and image reconstruction
I have a question about the operation of the optical microscope. With reference to the image:
from what I know, the image of the object is formed the first time where the red arrow is, why then is ...
- 941
0
votes
1
answer
170
views
In a Mirau Interferometer what is the distance between the mirror and the beamsplitter?
A Mirau interferometer (shown left in the figure, next to the Michelson configuration to the right) is an important instrument for non-contact surface profilometry.
Briefly, taken from Wikipedia, &...
- 611
0
votes
1
answer
252
views
Formula for total magnification of a compound microscope
In the book 'Materials Characterization: Introduction to Microscopic and Spectroscopic Methods, Second Edition pdf' its says:
The total magnification of a compound microscope as shown in Figure 1.1 ...
- 187
0
votes
0
answers
56
views
Could a reflective beam expander deteriorate the lateral symmetry of a beam collimated from a single-mode optical fiber?
Background: I am currently concerned with getting a confocal time-correlated single-photon counting (TCSPC) setup ready for autocorrelation spectroscopy (fundamentally: fluorescence autocorrelation (...
2
votes
0
answers
1k
views
Minimum Tube length distance between objective lens to tube lens
The maximum tube length between objective lens → tube lens (reference here)
MaxTubeLength = (TubeLensPupilDia-ObjectiveExitPupilDia) x (TubeLens_FL/SensorDiagonal)
You can use the calculator here. ...
- 21
0
votes
1
answer
100
views
Magnification of a system of lenses
I have a lens system consisting of 7 elements. However, I cannot take the system apart to measure the distance between them or their focal lengths. I do, however, know the effective focal length of ...
- 11
1
vote
1
answer
480
views
Magnification of a compound microscope
Whilst finding the magnification of a compound microscope, we multiply the linear magnification of the objective with the angular magnification of the eyepiece. This gives us the angular magnification ...
- 15
1
vote
1
answer
321
views
Regarding measurements on traveling microscope
In a experiment with traveling microscope to determine thickness of a glass slab , what is the physical significance of the readings that we get from the vertical scale ?, Means What are two points ...
1
vote
1
answer
36
views
Range of magnification outside plane of focus in a microscope
This should be a simple question, but somehow it's stumping me due to the way microscope objectives are, necessarily, built and classified (compared to, e.g., ideal thin lenses and photographic lenses)...
- 213
-1
votes
1
answer
386
views
How to draw ray diagrams for a compound microscope
I am trying to draw the ray diagram of a compound microscope. Here is my attempt (sorry if the image is kind of blurry):
I believe that I understand how the objective lens creates a real image (I ...
0
votes
1
answer
27
views
Microscopes magnification clarification
Why is the linear magnification $m_{obj}$ defined as
$\frac{-d_i^{obj}}{d_o^{obj}}$?
- 409
1
vote
1
answer
47
views
Conjugation relation of an oil immersion objective
I can not find out the right conjugation relation for an oil immersion objective (oil in the object space, air in the image space).
If I send an incident plane wave in air with angle $\alpha_{air}$ ...
- 23