All Questions
Tagged with synthesis biochemistry
16
questions
3
votes
4
answers
622
views
Is there a heat-based alternative to photosynthesis?
I can imagine one main disadvantage of a process using sunlight to metabolize sugars would be that wherever there is little/no sun (nighttime), plants don't grow. Heat is more abundant than sunlight ...
- 43
4
votes
1
answer
164
views
What is the maximum length of peptide which considered to be hard for synthesis by the current technology?
I am a molecular biologist and work with recombinant proteins. I am wondering what the cut point, in terms of length, is at which I should produce the peptide using heterologous expression rather than ...
- 131
7
votes
1
answer
159
views
In solid-phase peptide synthesis, why are the formation of aspartimides a problem but not "glutamides"?
I'm studying solid-phase peptide synthesis (SPPS) at the moment, and a common problem is the formation of aspartimides, which disrupt the synthetic process.
I understand how they form and why are they ...
- 71
5
votes
1
answer
44
views
Carbon 11 radiolabeled inhaled Beta-2 agonist or corticosteroids, is it possible?
Background
Positron emission tomography (PET) is a highly sensitive imaging modality that can provide in vivo quantitative information of biological processes at a biochemical level. PET relies upon ...
- 51
2
votes
0
answers
87
views
Which amino acids can be totally synthesized? [closed]
I can't find information regarding which amino acids (say the proteinogenic ones or in general) can be totally synthesized. Is there a reference?
- 37
0
votes
0
answers
92
views
Synthesis question: Using reductive amination to form pyrrolidine
What molecule results from treating 4-oxopentanal with $\ce{NH4Cl}$ and $\ce{NaCNBH3}$? Why? Is this a good, high yield reaction?
I believe that pyrrolidine is formed because amination will convert ...
- 1
1
vote
0
answers
36
views
Dispersity of synthetic DNA [closed]
Recently I was reading an article about DNA nanostructures and I was wondering--
I know that DNA in living systems is monodisperse, but when we make DNA synthetically, is it truly monodisperse or ...
- 11
1
vote
0
answers
74
views
Selective Esterification of Amino Acid
I am going to prepare tert-butyl protected threonine. I was going for the classic esterification methodology which is acid catalyzed (sulfuric acid) esterification under reflux of tert butyl alcohol + ...
- 11
3
votes
1
answer
99
views
Purification of Solid Phase Synthesized Peptide
I began working in a newly setup lab, and I'm having a dilysine (K2) peptide synthesized by solid phase method. I plan to cleave the peptides from the resin with a cleavage cocktail of TFA. ...
- 133
4
votes
1
answer
524
views
Why is molybdenum that important in inorganic synthesis as well as biosynthesis of ammonia?
We know that Mo (Molybdenum ) is used as a catalyst for Haber's process, during synthesis of Ammonia from Nitrogen. Again, in biological systems, Nitrogen fixation (ALSO forming Ammonia from Nitrogen)...
user73099
-1
votes
1
answer
142
views
Opening the imidazoline ring in tolazoline
Hello.
I was studying about the alkaline ring opening or purines. While doing research, i came across this compound. This got me thinking if we can use the same procedure to open the imidazoline ring ...
- 61
2
votes
0
answers
49
views
Can we create human muscles artificially?
Can chemical processes create sarcomeres? Not the entire cell with mitochondria, just what basically makes the muscle contract, myosin and actin, in the goal of reproducing our muscles. The source of ...
- 21
5
votes
1
answer
4k
views
Why does capping after each coupling in Solid phase peptide synthesis improve the results?
Why does capping after coupling improve results during solid phase peptide synthesis (SPPS)?
The Case: I have conducted a lab experiment where we coupled three Fmoc-protected amino acids, and did a ...
- 918
13
votes
3
answers
5k
views
Why is solid phase peptide synthesis limited to 70 amino acids?
I'm reading document about Solid phase protein synthesis (SPPS) from Wikipedia, and according to the document:
SPPS is limited by yields, and typically peptides and proteins in the
range of 70 ...
- 727
8
votes
1
answer
2k
views
How is Melatonin Synthesised?
I should like to know roughly how the hormone melotonin is synthetically made for the pharmaceutical industry (i.e. not how it is made in living beings). I can't seem to find any reference to its ...
- 241