pH= 8 Absorbance = 0.997 Concentration mol/l = 6.01E-05
In this experiment I used Beer's law to convert absorbance to concentration with a known extinction coefficient (16.6*10^3) - so 0.997/16600 = concentration.
Next I want to find the amount of solute in the solution, which is that triangle formula, Amount in moles (mol) = concentration (mol/l) / volume (l), with a given volume of 8ml.
There are two stipulations: the given volume was 8ml not 8L, and I should be presenting the 'amount in moles' as micromoles. So 6.01E-05 moles is equal to 60.06 µmol, but it is still in µmol/L, whereas I want it µmol/ml. Therefore I multiply by 1000 again, giving 60060 µmol/ml.
Then to find Amount in moles using that triangle thing, I would multiply 60060 by 0.008 - right? This gives 480.48 moles, however I need µmol, so it's 480481 µmol?
This really seems wrong and I would appreciate some help!