I want to perform chemical stability studies for different pH Values. To achieve pH Values from 1 to 6 I can prepare solutions with trifluoric acid and formic acid and ammonium formate and acetate. I know that the very high and low values are outside the optimal buffer range, but it still works because the sample concentration is small compared to an high excess of testing buffer.
For the range of 7 to 12 I can achieve these values by mixing ammonium bicarbonate and ammonium hydroxide in specific quantities. Still, I am wondering if there is a way to also test the high pH range 12-14 with a system that is LCMS compatible. Normally these pH ranges are accessed by using sodium hydroxide. But this is not compatible with LCMS, because hard ions are harmfull for mass detectors. I know that normally silica columns for rp18 are used in the pH 2-8 range. The solutions are therefore obviously diluted with LCMS mobile phase that contains formic acid before they are injected in the systems to achieve an acceptable pH value and sample concentration. Still, sodium formate would not be volatile. I know, that it is possible to divert the first minutes of an hplc run into the waste before they enter the mass detector. Still, if your product is being deteriorated into very polar substances (which I suspect for my case), you would not observe these species. Is there a possibility to access these pH values with a volatile reagent that is not harmfull for LCMS detectors? Alternatively I am thinking of doing NMR testing, still here you would be limited to heteroatoms to avoid water contamination in the spectra and you can not time the measurements as flexible as LCMS runs.