Yes, weird plant scientist asking weird plant questions...
Background: I am looking at how the twigs of trees absorb water, a poorly understood means by which trees alleviate water stress. Of 4 fluorescent tracers in an aqueous solution, experimentally applied to twig surfaces, 2 enter the twigs and 2 do not. The 2 that are absorbed have a hydrogen bond donor count that is 6-8X greater than the 2 that don't. Of all the chemical properties, this is by far the most different between the pairs. Oddly, it is the largest and smallest molecules that are absorbed, so I am thinking it is not molecular weight. All 4 compounds have a formal charge of zero.
The solutions were applied directly to the intact surface of detached (but live) twigs, on the twig epidermis (not leaves), during the day. Transport was detected throughout, both symplastically (inside the cells so a membrane was crossed) and apoplastically (outside the membrane, so a layer of suberin was crossed).
The apoplastic tracers were: Sulforhodamine B sodium salt (not absorbed) and Calcofluor white (absorbed)
The symplastic tracers were: HPTS (not absorbed) and 5(6)-Carboxyfluorescein (absorbed)
Question: What does it mean? Can an H-bond donor count positively influence absorption, particularly across suberin/other waxes or membranes (the 2 possible entry points)? Or could the smaller HBD:HBA ratio impact absorption? How?
Thanks for any insight! :)