I stained my work station with Coomassie blue solution during my SDS-PAGE protocol. I have been cleaning but the solution seems to can't be removed. What should I use to remove all these stains?
6
-
$\begingroup$ What is the surface of the work-station made of? How long have the stains been there? What are you using to clean the stains? $\endgroup$– anantaCommented May 31, 2023 at 10:19
-
1$\begingroup$ What is a SDS-PAGE protocol ? $\endgroup$– MauriceCommented May 31, 2023 at 12:07
-
$\begingroup$ Coomassie Blue is colorless at pH 1 - 3. So by adding a $\ce{HCl}$ solution diluted to about 0.01 M, it should make the blue dye colorless. $\endgroup$– MauriceCommented May 31, 2023 at 12:14
-
4$\begingroup$ The color will come back once HCl evaporates. A very dilute solution of bleach (used as per manufacturer's and lab instructions) should remove dye stains. $\endgroup$– ACRCommented May 31, 2023 at 12:34
-
$\begingroup$ Use organic solvents, like ethanol, DMSO, etc. $\endgroup$– anantaCommented May 31, 2023 at 14:23
|
Show 1 more comment