I hope everyone is doing well and safe! Yesterday, I was fortunate enough to be taught about how to read a chromatography calibration curve through this stackexchange! Special thanks to those who explained to me patiently and meticulously. Anyways, I've tried to solve some questions regarding this field but ended up no-where with this one. Could someone run me through the procedure of how to solve this question? Huge thanks in advance!!! <3 <3
Info: A $\pu{1.0mL}$ aliquot of a drink was diluted to $\pu{50.0mL}$ with deionized water. A sample of the drink was run through the HPLC to produce the calibration curve below. In this experiment, the mass of caffeine in the drink was determined by HPLC. The peak area obtained for this diluted sample was $2800$ arbitrary units.
Question: What is the mass of caffeine in grams, in a $\pu{330mL}$ can of this drink?
Answer = $\pu{825mg}$
P.S.: Could someone explain to me the significance of the peak area ($2800$ arbitrary units) that is obtained? And thank you for the constant support and help. Much love to this community!!!