All Questions
5
questions
0
votes
2
answers
202
views
How do you set the coverage in PacBio's Sequel II?
I am reading the Whole Genome Sequencing for de novo Assembly Best Practices
Use the Sequel II or IIe System and SMRT® Cell 8M to sequence to desired coverage depth for complexity
of genome
10- to 15-...
- 280
3
votes
2
answers
278
views
Contamination on genome assembly
I had a question for the community.
I have a genome of a new species that has been sequenced via 150pb Illumina paired-end.
To verify the quality of the assembly I used the ...
- 199
2
votes
1
answer
237
views
Coverage required
I was came across a problem during an exercise in a book and I don't really know how to solve it. I feel like something's missing.
"coverage, c = $NL/G$ (N=number of reads, L=read length, G=genome ...
- 21
1
vote
1
answer
73
views
How many reads do I need for hybrid assembly
I have Illumina and PacBio reads and I would like to use dbg2olc for hybrid assembly.
Part of dbg2olc is SelectLongestReads which select reads that sum to ...
- 453
7
votes
1
answer
295
views
How to calculate overall reference coverage with MUMmer?
Is the MUMmer suite capable of calculating reference sequence coverage statistics for all query sequences collectively? It would be possible to achieve by parsing the output of ...
- 248