Assessing the quality of an assembly

Question

I am trying to run a script that assess the quality of a transcriptomic assembly, a de novo assembly using a tool called Transrate. To install the tool I followed the prompts in https://bioconda.github.io/recipes/transrate/README.html I work on an HPC therefore I used module load mamba/23.3.1-0 created a mamba environment and called it transrate, I installed the tool using mamba install transrate and then I activated the environment called transrate using  mamba activate transrate. When I use mamba list it shows me its dependencies and when I use gem list I can see that transrate has been installed. On my bash script this is what I have:

module load mamba/23.3.1-0
# Activate the Conda environment
mamba activate transrate
echo "Assembly assessment with Transrate"
transrate --assembly /path/to/my/assembly/Trinity.fasta --left trimmed_SRR609893_1.fastq.gz --right trimmed_SRR609893_2.fastq.gz --threads 8 --output /path/to/my/output/directory/
echo "Assement complete" 

When I submit this job in a bash script using sbatch transrate.sh on my cluster I get an error log file which shows me this:

Run 'mamba init' to be able to run mamba activate/deactivate
and start a new shell session. Or use conda to activate/deactivate.

/var/lib/slurm/slurmd/job9637707/slurm_script: line 15: transrate: command not found

I don't know what the issue is because when I use the mamba init it says no changes done, and I am still able to use mamba activate or deactivate. I do not understand why it says command transrate is not found yet on that environment it is present when using "gem list".

May I please get assistance on the matter, ,or get an alternative that I can use to assess a de novo assembly.