CMFM Centre Satellite Symposium-Workshop
If you’d like to attend please click “register now” or email us at CMFM@imperial.ac.uk (limited places).
Program:
Part 1 Symposium
Session1. Cardiovascular mechanosensation session
8:45-9:15 Arrival, tea and coffee
9:15-9:20 Prof. Julia Gorelik – Director of Multi-faculty Centre for Cellular Mechanosensing and Functional Microscopy (CMFM)
Welcome and Introduction
9:20-9:35 Prof. Thomas Iskratsch (Queen Mary University of London’s School of Engineering and Materials Science, UK)
“Methods to analyse cardiomyocyte mechanosensing and mechanical memory from single receptor to cellular scale.”
9:35-9:50 Prof Michele Miragoli (University of Parma and Humanitas Research Hospital, IT)
“High-throughput kinematic screening for cardiac organoids with impairment in contraction”
9:50-10:05 Prof. Katja Odening, (University of Bern, CH)
“Mechanoelectrical feedback in the ‘electrical’ diseases long-QT and short-QT syndrome”
10:05-10:20 Prof. Ruiz Lozano-Pilar (Regencor INC and Imperial College London, UK)
“Alternatives for Pharmaceutical delivery of Biologics”
10:20-10:35 Dr. Guillermo Luxan (Dimmeler Lab, Institute of Cardiovascular Regeneration, Frankfurt, Germany)
“Extracellular matrix and cardiac ageing”
10:35-10:50 Dr Thomas Qvistgaard Jepps, (Copenhagen University, Denmark)
“The microtubule network of vascular smooth muscle cells in hypertension”
11:00-11:30 Coffee break
Session 2. New technologies and models for cardiovascular biology
11:30-11:45 Dr Andrew Shevchuk (Department of Metabolism, IC)
“Scanning Ion Conductance Microscopy applications”.
11:45-12:00 Dr Pamela Swiatlowska (NHLI, IC)
”SICM as a novel tool to measure cardiomyocyte Nanomechanics”
12:00-12:15 Prof Cesare Terracciano (NHLI, IC)
“Physiological control of mechanical load in vitro: living myocardial slices and micro-vascularised human engineered cardiac tissue”.
12:15-12:30 Dr Graeme M Birdsey (NHLI, IC)
“Lymphatic endothelial cells forming 3D sprouts in a novel modified gelatin hydrogel system”.
12.30-12.40 Q&A session
12:40-13:30 Lunch Break
Part 2 Workshop
13:30-17:00 Practical demonstration – 30-40 minutes rotations
A. Dr Graeme M Birdsey and Prof Anna Randi laboratories – Organ-on-chip technology
1. Lymphatic endothelial cells forming 3D sprouts in a novel modified gelatin hydrogel system
2. Lymphatic endothelial cell 3D networks in a stretching device to mimic cardiac muscle contraction.
3. Co-culture of endothelial cells and cardiomyocytes in an organ-on-chip platform
B. Prof. Cesare Terracciano’s laboratory – Living Myocardial Slices
1. Preparations of myocardial slices
2. Using the bioreactor for the cultivation of myocardial slices
3. Measurements of contraction and calcium dynamics from myocardial slices
4. Stretching devices for studying the loading of myocardial slices
C. Prof. Yuri Korchev’s laboratory (Dr. Andrew Shevchuk and Dr. Petr Gorelkin)- different applications of SICM and other combinations of SICM techniques for mechanobiological applications
1. SICM topography and Young’s modulus mapping of the endothelial and neuronal cells.
2. Correlative 3D microscopy of single cells using confocal and scanning ion-conductance microscopy.
3. SICM probe as a tool for ROS, O2, and pH measurements on single cells and organoids.
4. SICM nanopipette as a tool for nanopore sensing technology.
D. Prof. Julia Gorelik laboratory (Dr. Jose L Sanchez Alonso-Mardones and Dr. Pamela Swiatlowska) – different applications of SICM and other combinations of SICM techniques for mechanobiological cardiovascular applications
1. Measurement of Young Modulus from live adult cardiomyocytes
2. Measurement of the stiffness of live neonatal cardiomyocytes in culture and co-culture with neurons.
3. FRET/SICM technique for studying cAMP compartmentation in live cells
4. Single-cell stretching in combination with SICM and FRET
17:00 End of the day, departure